Achieving rapid low-pressure ion chromatography separations on short silica-based monolithic columns

被引：39

作者：

Pelletier, Sarah ^{[1
]}

Lucy, Charles A. ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Gunning Lemieux Chem Ctr, Edmonton, AB T6G 2G2, Canada

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1118卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

ion chromatography; short monolithic columns; fast separations; anions; extra-column band broadening; coating stability; flow injection analysis (FIA);

D O I：

10.1016/j.chroma.2006.03.072

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Short silica-based monolithic columns (0.5-1 cm) are coated with the surfactant didodecyldimethylammonium bromide (DDAB) and used for fast ion exchange separations of small inorganic anions. Sources of extra-column band broadening are assessed and minimized to obtain separations of seven analytes (iodate, chloride, nitrate, bromide, nitrite, phosphate, sulphate) in two minutes at 2 mL/min. Eluents used are 6 and 9 mM 4-cyanophenol at pH 7.3-7.4 or 5 mM 4-hydroxybenzoic acid at pH 5.6. DDAB coating stability is improved by 15-fold by the addition of a DDAB coated pre-column before the injection valve. Separations are obtained using a low-pressure glass syringe. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：12 / 18

页数：7