Isoform specific changes in PPARα and β in colon and breast cancer with differentiation

被引：28

作者：

Aung, CS ^{[1
]}

Faddy, HM ^{[1
]}

Lister, EJ ^{[1
]}

Monteith, GR ^{[1
]}

Roberts-Thomson, SJ ^{[1
]}

机构：

[1] Univ Queensland, Sch Pharm, Brisbane, Qld 4072, Australia

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 340卷 / 02期

基金：

英国医学研究理事会;

关键词：

colon; breast; differentiation; cell lines; RT-PCR; PPAR; human; sodium butyrate; post-confluence; alkaline phosphatase intestinal;

D O I：

10.1016/j.bbrc.2005.12.061

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the role of peroxisome proliferator-activated receptors (PPARs) chi and beta in the differentiation of colon cancer cells, we differentiated HT-29 cells using sodium butyrate (NaB) and culturing post-confluence and assessed differentiation using the marker intestinal alkaline phosphatase. While PPAR chi levels only changed with culturing post confluence, PPAR beta levels increased independent of the method of differentiation. To explore further the differences induced by NaB. we assessed changes in both PPAR isoforms in MCF-7 breast cancer cells cultured in the presence of NaB over 48 h. Again a very different expression pattern was observed with PPAR-1 increasing after 4 h and remaining elevated, while PPAR beta increased transiently. Our studies suggest that the expression of PPARs is dependent upon both the method of differentiation and on time. Moreover, these studies show that changes in levels are not required for the differentiation of colon cancer cell lines, whereas changes in PPAR beta are more closely associated with differentiation. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：656 / 660

页数：5

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