Differential distribution of apolipoprotein E in young and aged spontaneously hypertensive and stroke-prone rats

被引:10
作者
Chiang, AN [1 ]
Chang, CP
Chou, YC
Huang, KY
Hu, HH
机构
[1] Natl Yang Ming Univ, Inst Biochem, Taipei 112, Taiwan
[2] Natl Yang Ming Univ, Inst Physiol, Taipei 112, Taiwan
[3] Vet Gen Hosp, Dept Neurol, Taipei, Taiwan
关键词
apolipoprotein E; high-density lipoprotein; low-density lipoprotein; very-low-density lipoprotein; cholesterol; triglyceride; hypertension; stroke;
D O I
10.1097/00004872-199917060-00011
中图分类号
R6 [外科学];
学科分类号
1002 ; 100210 ;
摘要
Objectives To evaluate whether the differential distribution of apolipoprotein E among lipoprotein fractions and hepatic expression of the apolipoprotein E gene are causal factors in the regulation of lipid metabolism and physiological functions in young and aged spontaneously hypertensive and stroke-prone rats. Design and methods Biochemical analyses were performed on serum and hepatic specimens from young (2-month-old) and aged (8-month-old) spontaneously hypertensive rats, stroke-prone spontaneously hypertensive rats and normotensive Wistar-Kyoto rats. Levels of apolipoprotein E among various lipoprotein fractions were determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Liver concentrations of apolipoprotein E mRNA were analyzed by Northern blotting and relative levels of apolipoprotein E mRNA in different strains of rats were compared. Statistical analysis was performed by measuring correlations between hepatic apolipoprotein E mRNA levels and biological parameters. Results Levels of apolipoprotein E in high-density and low-density lipoproteins were significantly lower in hypertensive rats than in age-matched normotensive Wistar-Kyoto rats. Although there was a significant increase in high-density lipoprotein apolipoprotein E contents in all aged animals, the elevation in aged hypertensive rats was much less than that in aged normotensive rats. Levels of apolipoprotein E in the very-low-density lipoprotein fraction were diminished in young stroke-prone rats but were remarkably high in aged rats. Steady-state levels of apolipoprotein E mRNA increased with age in all strains of rats, whereas aged hypertensive rats exhibited lower apolipoprotein E mRNA levels than aged normotensive rats. Conclusions The distribution of apolipoprotein E among various lipoprotein fractions was dramatically altered with age, and the alteration varied among different strains of rats. The differential distribution of apolipoprotein E in young and aged spontaneously hypertensive and stroke-prone rats suggests that apolipoprotein E could be a causal factor that disturbs the homeostasis of lipids and lipoproteins and perturbs physiological functions in hypertensive rats. J Hypertens 1999, 17:793-800 (C) Lippincott Williams & Wilkins.
引用
收藏
页码:793 / 800
页数:8
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