A review of chromatographic methods for the assessment of phospholipids in biological samples

被引：199

作者：

Peterson, BL ^{[1
]}

Cummings, BS ^{[1
]}

机构：

[1] Univ Georgia, Coll Pharm 371, Dept Pharmaceut & Biomed Sci, Athens, GA 30602 USA

来源：

BIOMEDICAL CHROMATOGRAPHY | 2006年 / 20卷 / 03期

关键词：

phospholipids; chromatography; mass spectrometry; matrix-assisted laser desorption ionization; electrospray ionization; lipidomics;

D O I：

10.1002/bmc.563

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phospholipids are important constituents of all living cell membranes. Lipidomics is a rapidly growing field that provides insight as to how specific phospholipids play roles in normal physiological and disease states. There are many analytical methods available for the qualitative and quantitative determination of phospholipids. This review provides a summary of the methods that were historically used such as thin layer chromatography, gas chromatography and high-performance liquid chromatography. In addition, an introduction to applications of interfacing these traditional chromatographic techniques with mass spectrometry is provided. Copyright (c) 2005 John Wiley & Sons, Ltd.

引用

页码：227 / 243

页数：17

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