Crystal structure of Cry51Aa1: A potential novel insecticidal aerolysin-type β-pore-forming toxin from Bacillus thuringiensis

被引：28

作者：

Xu, Chengchen ^{[1
,2
]}

Chinte, Unmesh ^{[2
,3
,4
]}

Chen, Lirong ^{[2
]}

Yao, Qingqing ^{[5
]}

Meng, Ying ^{[1
]}

Zhou, Dayong ^{[2
]}

Bi, Li-Jun ^{[6
]}

Rose, John ^{[2
,3
,4
]}

Adang, Michael J. ^{[2
,7
]}

Wang, Bi-Cheng ^{[2
,3
,4
]}

Yu, Ziniu ^{[1
]}

Sun, Ming ^{[1
]}

机构：

[1] Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China

[2] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA

[3] Univ Georgia, SER CAT, Argonne, IL 60439 USA

[4] Argonne Natl Lab, Adv Photon Source, Argonne, IL 60439 USA

[5] Fudan Univ, Sch Life Sci, Dept Biochem, Shanghai 200438, Peoples R China

[6] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

[7] Univ Georgia, Dept Entomol, Athens, GA 30602 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2015年 / 462卷 / 03期

关键词：

Bacillus thuringiensis; Crystal structure; Insecticidal; Pore-forming toxin; PROTEIN; SIMILARITY; PARASPORIN; BINDING;

D O I：

10.1016/j.bbrc.2015.04.068

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structures of several Bacillus thuringiensis (Bt) insecticidal crystal proteins have been determined by crystallographic methods and a close relationship has been explicated between specific toxicities and conserved three-dimensional architectures. In this study, as a representative of the coleopteran- and hemipteran-specific Cry51A group, the complete structure of Cry51Aa1 protoxin has been determined by X-ray crystallography at 1.65 angstrom resolution. This is the first report of a coleopteran-active Bt insecticidal toxin with high structural similarity to the aerolysin-type beta-pore forming toxins (beta-FFTs). Moreover, study of featured residues and structural elements reveal their possible roles in receptor binding and pore formation events. This study provides new insights into the action of aerolysin-type (beta-PFTs from a structural perspective, and could be useful for the control of coleopteran and hemipteran insect pests in agricultures. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：184 / 189

页数：6

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