Catalpol inhibits apoptosis in hydrogen peroxide-induced PC12 cells by preventing cytochrome c release and inactivating of caspase cascade

被引：93

作者：

Jiang, B ^{[1
]}

Liu, JH ^{[1
]}

Bao, YM ^{[1
]}

An, LJ ^{[1
]}

机构：

[1] Dalian Univ Technol, Dept Biochem Engn, Dalian 116024, Peoples R China

来源：

TOXICON | 2004年 / 43卷 / 01期

关键词：

catalpol; hydrogen peroxide; apoptosis; PC12; cells; caspase; cytochrome c;

D O I：

10.1016/j.toxicon.2003.10.017

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In the present study, using a rat pheochromocytoma (PC12) cell line, the effect of catalpol on H2O2-induced apoptosis was studied. The apoptosis in H2O2-induced PC12 cells was accompanied by down-regulation of Bcl-2, up-regulation of Bax, the release of mitochondrial cytochrome c to cytosol and sequential activation of caspase-1 and caspase-3 then leading to cleavage of poly-ADP-ribose polymerase (PARP). Catalpol not only suppressed the down-regulation of Bcl-2, up-regulation of Bax and the release of mitochondrial cytochrome c to cytosol, but also attenuated caspase-3 activation, PARP cleavage, and eventually protected against H2O2-induced apoptosis. Taken together, these results suggest that treatment of PC 12 cells with catalpol can block H2O2-induced apoptosis by the regulation of Bcl-2 family members, as well as suppression of cytochrome c release and caspase cascade activation. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：53 / 59

页数：7

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