Netrin UNC-6 and the regulation of branching and extension of motoneuron axone from the ventral nerve gord of Caenorhabditis elegans

被引:36
作者
Lim, YS [1 ]
Mallapur, S [1 ]
Kao, GT [1 ]
Ren, XC [1 ]
Wadsworth, WG [1 ]
机构
[1] Robert Wood Johnson Med Sch, Dept Pathol, Piscataway, NJ 08854 USA
关键词
netrin; UNC-6; C; elegans; guidance; axon branching; genetics; invertebrate; in vivo;
D O I
10.1523/JNEUROSCI.19-16-07048.1999
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In the Caenorhabditis elegans embryo, some ventral midline motoneurons extend a process circumferentially to the dorsal midline and a process longitudinally along ventral nerve cord interneurons. Circumferential migrations are guided by netrin UNC-6, which repels motoneuron axons dorsally. Although the motoneuron cell bodies and the longitudinal axons are positioned along UNC-6-expressing interneurons in the ventral nerve cord, the circumferential processes extend only from the motoneuron cell bodies and from defined locations along some longitudinal axons. This implies a mechanism regulates motoneuron branching of UNC-6-responsive processes. We show that expression of unc-6 Delta C, which encodes UNC-6 without domain C, partially rescues circumferential migration defects in unc-6 null animals. This activity depends on the netrin receptors UNC-5 and UNC-40. These results indicate that UNC-6 Delta C can provide the circumferential guidance functions of UNC-6. Furthermore, we show that expression of unc-6 Delta C causes motoneuron branching and the extension of processes from abnormal positions along the ventral nerve cord. This activity is also UNC-5- and UNC-40-dependent, We propose that local interactions mediated by domain C regulate motoneuron branching and responsiveness to the UNC-6 cue.
引用
收藏
页码:7048 / 7056
页数:9
相关论文
共 51 条
[11]   Guidance cues at the Drosophila CNS midline: Identification and characterization of two Drosophila Netrin/UNC-6 homologs [J].
Harris, R ;
Sabatelli, LM ;
Seeger, MA .
NEURON, 1996, 17 (02) :217-228
[12]   SYNAPTIC CODE FOR SENSORY MODALITIES REVEALED BY C-ELEGANS GLR-1 GLUTAMATE-RECEPTOR [J].
HART, AC ;
SIMS, S ;
KAPLAN, JM .
NATURE, 1995, 378 (6552) :82-85
[13]   THE UNC-5, UNC-6, AND UNC-40 GENES GUIDE CIRCUMFERENTIAL MIGRATIONS OF PIONEER AXONS AND MESODERMAL CELLS ON THE EPIDERMIS IN C-ELEGANS [J].
HEDGECOCK, EM ;
CULOTTI, JG ;
HALL, DH .
NEURON, 1990, 4 (01) :61-85
[14]  
HEKIMI S, 1993, J NEUROSCI, V13, P4254
[15]   UNC-6, A LAMININ-RELATED PROTEIN, GUIDES CELL AND PIONEER AXON MIGRATIONS IN C-ELEGANS [J].
ISHII, N ;
WADSWORTH, WG ;
STERN, BD ;
CULOTTI, JG ;
HEDGECOCK, EM .
NEURON, 1992, 9 (05) :873-881
[16]   Deleted in Colorectal Cancer (DCC) encodes a netrin receptor [J].
KeinoMasu, K ;
Masu, M ;
Hinck, L ;
Leonardo, ED ;
Chan, SSY ;
Culotti, JG ;
TessierLavigne, M .
CELL, 1996, 87 (02) :175-185
[17]   NETRINS ARE DIFFUSIBLE CHEMOTROPIC FACTORS FOR COMMISSURAL AXONS IN THE EMBRYONIC SPINAL-CORD [J].
KENNEDY, TE ;
SERAFINI, T ;
DELATORRE, JR ;
TESSIERLAVIGNE, M .
CELL, 1994, 78 (03) :425-435
[18]   frazzled Encodes a Drosophila member of the DCC immunoglobulin subfamily and is required for CNS and motor axon guidance [J].
Kolodziej, PA ;
Timpe, LC ;
Mitchell, KJ ;
Fried, SR ;
Goodman, CS ;
Jan, LY ;
Jan, YN .
CELL, 1996, 87 (02) :197-204
[19]   THE CAENORHABDITIS-ELEGANS ROL-6 GENE, WHICH INTERACTS WITH THE SQT-1 COLLAGEN GENE TO DETERMINE ORGANISMAL MORPHOLOGY, ENCODES A COLLAGEN [J].
KRAMER, JM ;
FRENCH, RP ;
PARK, EC ;
JOHNSON, JJ .
MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (05) :2081-2089
[20]   Axon tracts correlate with Netrin-1a expression in the zebrafish embryo [J].
Lauderdale, JD ;
Davis, NM ;
Kuwada, JY .
MOLECULAR AND CELLULAR NEUROSCIENCE, 1997, 9 (04) :293-313