Improved detection of apoptotic cells using a modified in situ TUNEL technique

被引:48
作者
Cuello-Carrión, FD [1 ]
Ciocca, DR [1 ]
机构
[1] Reg Ctr Sci & Technol Res, Lab Reprod & Lactat, Endocrine Pathol Unit, Mendoza, Argentina
关键词
apoptosis; TUNEL; sensitivity;
D O I
10.1177/002215549904700614
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We are in the process of assessing the response of cancer tissues to chemotherapy, evaluating, among other points, the proportion of cancer cells undergoing apoptosis. However, the apoptotic index obtained with the original TUNEL technique was lower than that obtained by evaluation of apoptosis on H&E-stained sections. Here we describe a small modification of the TUNEL technique that significantly increases the sensitivity of the assay. In the nonmodified TUNEL technique, a digoxigenin-labeled probe is detected using a direct peroxidase-conjugated system, whereas here we report the advantage of using a streptavidin-biotin-immunoperoxidase system. This, in conjunction with pretreatment of tissue sections with proteinase K and microwave irradiation, improved the detection of apoptotic cells.
引用
收藏
页码:837 / 839
页数:3
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