A polymerase chain reaction protocol for the detection of various geographical isolates of white spot virus

被引：55

作者：

Tapay, LM

Nadala, ECB

Loh, PC

机构：

[1] Univ Hawaii, Dept Microbiol, Honolulu, HI 96822 USA

[2] Univ Philippines, Los Banos Coll, Natl Inst Mol Biol & Biotechnol, Laguna, Philippines

来源：

JOURNAL OF VIROLOGICAL METHODS | 1999年 / 82卷 / 01期

关键词：

PCR; one-step; two-step; WSV-DNA; detection;

D O I：

10.1016/S0166-0934(99)00081-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polymerase chain reaction (PCR) primers were designed based on the sequence of a cloned fragment of the white spot virus (WSV) genome and were used to detect at least four geographic isolates of WSV from both experimentally- and naturally-infected shrimp. In addition to high specificity, the one-step and two-step PCR protocols were determined to have sensitivities of 10-100 pg and 100 femtograms respectively. The two-step PCR protocol is recommended as a very sensitive and specific alternative protocol to Western blot assay for the detection of WSV. (C) 1999 Elsevier Science B.V. All rights reserved.

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收藏

页码：39 / 43

页数：5

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