Pathway of cytosolic starch synthesis in the model glaucophyte Cyanophora paradoxa

被引：31

作者：

Plancke, Charlotte ^{[1
]}

Colleoni, Christophe ^{[1
]}

Deschamps, Philippe ^{[1
]}

Dauvillee, David ^{[1
]}

Nakamura, Yasunori ^{[2
]}

Haebel, Sophie ^{[3
]}

Ritte, Gehrardt

Steup, Martin

Buleon, Alain ^{[4
]}

Putaux, Jean-Luc ^{[5
]}

Dupeyre, Danielle ^{[5
]}

d'Hulst, Christophe ^{[1
]}

Ral, Jean-Philippe ^{[1
]}

Loeffelhardt, Wolfgang ^{[6
]}

Ball, Steven G. ^{[1
]}

机构：

[1] Univ Sci & Tech Lille Flandres Artois, CNRS USTL, UMR8576, Unite Glycobiol Struct & Fonct, F-59655 Villeneuve Dascq, France

[2] Akita Prefectural Univ, Fac Bioresource Sci, Akita 0100195, Japan

[3] Univ Potsdam, Ctr Mass Spectrometry Biopolymers, Inst Biochem & Biol, D-14476 Potsdam, Germany

[4] Ctr Rech Agroalimentaires, Inst Natl Rech Agron, F-44316 Nantes 03, France

[5] Ctr Rech Macromol Vegetales CERMAV CNRS, F-38041 Grenoble 9, France

[6] Univ Vienna, Dept Biochem, Max F Perutz Labs, A-1030 Vienna, Austria

来源：

EUKARYOTIC CELL | 2008年 / 7卷 / 02期

关键词：

D O I：

10.1128/EC.00373-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The nature of the cytoplasmic pathway of starch biosynthesis was investigated in the model glaucophyte Cyanophora paradoxa. The storage polysaccharide granules are shown to be composed of both amylose and amylopectin fractions, with a chain length distribution and crystalline organization similar to those of green algae and land plant starch. A preliminary characterization of the starch pathway demonstrates that Cyanophora paradoxa contains several UDP-glucose-utilizing soluble starch synthase activities related to those of the Rhodophyceae. In addition, Cyanophora paradoxa synthesizes amylose with a granule-bound starch synthase displaying a preference for UDP-glucose. A debranching enzyme of isoamylase specificity and multiple starch phosphorylases also are evidenced in the model glaucophyte. The picture emerging from our biochemical and molecular characterizations consists of the presence of a UDP-glucose-based pathway similar to that recently proposed for the red algae, the cryptophytes, and the alveolates. The correlative presence of isoamylase and starch among photosynthetic eukaryotes is discussed.

引用

页码：247 / 257

页数：11

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