Loss of Occludin Affects Tricellular Localization of Tricellulin

被引:151
作者
Ikenouchi, Junichi [1 ,6 ]
Sasaki, Hiroyuki [2 ]
Tsukita, Sachiko [3 ,4 ]
Furuse, Mikio [5 ]
Tsukita, Shoichiro [1 ]
机构
[1] Kyoto Univ, Fac Med, Dept Cell Biol, Kyoto 6068501, Japan
[2] Jikei Univ, Sch Med, Inst DNA Med, Dept Mol Cell Biol, Tokyo 1058461, Japan
[3] Osaka Univ, Biol Sci Lab, Grad Sch Frontier Biosci, Suita, Osaka 5650871, Japan
[4] Osaka Univ, Biol Sci Lab, Grad Sch Med, Suita, Osaka 5650871, Japan
[5] Kobe Univ, Grad Sch Med, Dept Physiol & Cell Biol, Div Cell Biol, Kobe, Hyogo 6500017, Japan
[6] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan
关键词
D O I
10.1091/mbc.E08-05-0530
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The tricellular tight junction (tTJ) forms at the convergence of bicellular tight junctions (bTJs) where three epithelial cells meet in polarized epithelia, and it is required for the maintenance of the transepithelial barrier. Tricellulin is a four transmembrane domain protein recently identified as the first marker of tTJ, but little is known about how tricellulin is localized at tTJs. As for the molecular mechanism of association of tricellulin with tight junctions (TJs), we found that tricellulin was incorporated into claudin-based TJs independently of binding to zona occludens-1. Unexpectedly, exogenous expression of tricellulin increased cross-links of TJ strands in the plasma membrane. As for the molecular mechanisms for localization of tricellulin at tricellular junctions, we found that knockdown of occludin caused mislocalization of tricellulin to bTJs, implying that occludin supports tricellular localization of tricellulin by excluding tricellulin from bTJs.
引用
收藏
页码:4687 / 4693
页数:7
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