A latex agglutination assay for specific detection of Panton-Valentine leukocidin

被引：12

作者：

Oishi, Kanenari ^{[2
]}

Baba, Tadashi

Nakatomi, Yasuo

Ito, Teruyo ^{[2
]}

Hiramatsu, Keiichi ^{[1
]}

机构：

[1] Juntendo Univ, Dept Bacteriol, Bunkyo Ku, Tokyo 1138421, Japan

[2] Denka Seiken Co Ltd, Bacterial Diagnost Prod Dept, Niigata 9591695, Japan

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2008年 / 75卷 / 03期

关键词：

Panton-Valentine leukocidin; Staphylococcus aureus; Reverse passive latex agglutination; Toxin producer strain;

D O I：

10.1016/j.mimet.2008.07.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Panton-Valentine leukocidin (PVL) is produced by some isolates of Staphylococcus aureus, and has been associated with the high pathogenicpotential of these strains. To rapidly detect the toxin producer strains, we developed a reverse passive latex agglutination (RPLA) reaction assay specific for PVL By testing 64 S. aureus strains, the assay could detect the 35 pvl-gene-positive strains with 100% specificity and sensitivity. Furthermore, the assay revealed an extensive variation in the amount of PVL produced by the pvl-positive strains. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：411 / 415

页数：5

共 34 条

[1] Genome and virulence determinants of high virulence community-acquired MRSA [J].

Baba, T ;

Takeuchi, F ;

Kuroda, M ;

Yuzawa, H ;

Aoki, K ;

Oguchi, A ;

Nagai, Y ;

Iwama, N ;

Asano, K ;

Naimi, T ;

Kuroda, H ;

Cui, L ;

Yamamoto, K ;

Hiramatsu, K .

LANCET, 2002, 359 (9320) :1819-1827

[2]

Bohach GA, 2000, GRAM-POSITIVE PATHOGENS, P367

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] CHARACTERIZATION OF STAPHYLOCOCCAL GAMMA-LYSIN [J].

CLYNE, M ;

BIRKBECK, TH ;

ARBUTHNOTT, JP .

JOURNAL OF GENERAL MICROBIOLOGY, 1992, 138 :923-930

[5]

COONEY J, 1988, J GEN MICROBIOL, V134, P2179

[6] THE GAMMA-HEMOLYSIN LOCUS OF STAPHYLOCOCCUS-AUREUS COMPRISES 3 LINKED GENES, 2 OF WHICH ARE IDENTICAL TO THE GENES FOR THE F AND S COMPONENTS OF LEUKOCIDIN [J].

COONEY, J ;

KIENLE, Z ;

FOSTER, TJ ;

OTOOLE, PW .

INFECTION AND IMMUNITY, 1993, 61 (02) :768-771

[7] Staphylococcus aureus bicomponent γ-hemolysins, HlgA, HlgB, and HlgC, can form mixed pores containing all components [J].

Dalla Serra, M ;

Coraiola, M ;

Viero, G ;

Comai, M ;

Potrich, C ;

Ferreras, M ;

Baba-Moussa, L ;

Colin, DA ;

Menestrina, G ;

Bhakdi, S ;

Prévost, G .

JOURNAL OF CHEMICAL INFORMATION AND MODELING, 2005, 45 (06) :1539-1545

[8] Rapid detection of Panton-Valentine leukocidin from clinical isolates of Staphylococcus aureus strains by real-time PCR [J].

Deurenberg, RH ;

Vink, C ;

Driessen, C ;

Bes, M ;

London, N ;

Etienne, J ;

Stobberingh, EE .

FEMS MICROBIOLOGY LETTERS, 2004, 240 (02) :225-228

[9] Widespread skin and soft-tissue infections due to two methicillin-resistant Staphylococcus aureus strains harboring the genes for Panton-Valentine leucocidin [J].

Diep, BA ;

Sensabaugh, GF ;

Somboona, NS ;

Carleton, HA ;

Perdreau-Remington, F .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (05) :2080-2084

[10] Panton-Valentine leukocidin:: A marker of severity for Staphylococcus aureus infection? [J].

Etienne, J .

CLINICAL INFECTIOUS DISEASES, 2005, 41 (05) :591-593

← 1 2 3 4 →