PACAP stimulates c-fos mRNAs in small cell lung cancer cells

被引：22

作者：

Draoui, M

Hida, T

Jakowlew, S

Birrer, M

Zia, F

Moody, TW

机构：

[1] NCI,BIOMARKERS & PREVENT RES BRANCH,ROCKVILLE,MD 20850

[2] GEORGE WASHINGTON UNIV,MED CTR,DEPT MICROBIOL,WASHINGTON,DC 20037

[3] GEORGE WASHINGTON UNIV,MED CTR,DEPT BIOCHEM,WASHINGTON,DC 20037

[4] GEORGE WASHINGTON UNIV,MED CTR,DEPT MOLEC BIOL,WASHINGTON,DC 20037

来源：

LIFE SCIENCES | 1996年 / 59卷 / 04期

关键词：

PACAP; PACAP antagonist; c-fos mRNA; nuclear oncogenes; small cell lung cancer;

D O I：

10.1016/0024-3205(96)00299-8

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The effects of PACAP on c-fos mRNA using small cell lung cancer (SCLC) cell was investigated. PACAP-27 (100 nM) increased c-fos mRNA 5-fold using NCI-N417 cells. The increase was concentration dependent with 0.1 nM PACAP-27 half maximally increasing c-fos mRNA. Also the increase in c-fos mRNA caused by PACAP was time dependent; being maximal after 1 hour and returning to basal values after 4 hours. PACAP-38 but not PACAP(28-38) increased c-fos mRNA. One uM PACAP(6-38), a PACAP receptor antagonist, inhibited the increase in c-fos mRNA caused by 1 nM PACAP. These data indicate that PACAP stimulates nuclear oncogene expression in SCLC cells.

引用

页码：307 / 313

页数：7