Identification of CARE-2-negative Candida albicans isolates as Candida dubliniensis

被引：32

作者：

Morschhäuser, J

Ruhnke, M

Michel, S

Hacker, J

机构：

[1] Univ Wurzburg, Zentrum Infekt Forsch, D-97070 Wurzburg, Germany

[2] Humboldt Univ, Virchow Klinikum, Charite, Berlin, Germany

来源：

MYCOSES | 1999年 / 42卷 / 1-2期

关键词：

Candida dubliniensis; CARE-2; fingerprinting; phylogeny; rRNA;

D O I：

10.1046/j.1439-0507.1999.00259.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Among 302 clinical yeast isolates originally identified as Candida albicans, we found 16 isolates that did not hybridize with the C. albicans-specific repetitive DNA element CARE-2. These 16 isolates produced abundant chlamydospores, did not grow at 43 degrees C, and exhibited a distinct randomly amplified polymorphic DNA profile. Sequence analysis of part of the 28S rDNA demonstrated that the CARE-2-negative isolates are not an atypical subgroup of C. albicans but belong to the recently described new species C. dubliniensis.

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页码：29 / 32

页数：4

相关论文

共 11 条

[1] Multiple molecular mechanisms contribute to a stepwise development of fluconazole resistance in clinical Candida albicans strains [J].

Franz, R ;

Kelly, SL ;

Lamb, DC ;

Kelly, DE ;

Ruhnke, M ;

Morschhäuser, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (12) :3065-3072

[2]

FRANZ R, 1999, IN PRESS MYCOSES

[3] MOLECULAR EPIDEMIOLOGY OF CANDIDA ISOLATES FROM AIDS PATIENTS SHOWING DIFFERENT FLUCONAZOLE RESISTANCE PROFILES [J].

LISCHEWSKI, A ;

RUHNKE, M ;

TENNAGEN, I ;

SCHONIAN, G ;

MORSCHHAUSER, J ;

HACKER, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (03) :769-771

[4] Standardized molecular typing of Candida albicans strains [J].

Lischewski, A ;

Harmsen, D ;

Hacker, J ;

Morschhauser, J .

MYCOSES, 1997, 40 (9-10) :369-372

[5] Most frequent scenario for recurrent Candida vaginitis is strain maintenance with ''substrain shuffling'': Demonstration by sequential DNA fingerprinting with probes Ca3, C1, and CARE2 [J].

Lockhart, SR ;

Reed, BD ;

Pierson, CL ;

Soll, DR .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (04) :767-777

[6] EPIDEMIOLOGY OF FUNGAL-INFECTIONS - THE PROMISE OF MOLECULAR TYPING [J].

PFALLER, MA .

CLINICAL INFECTIOUS DISEASES, 1995, 20 (06) :1535-1539

[7] Use of specialised isolation media for recognition and identification of Candida dubliniensis isolates from HIV-infected patients [J].

Schoofs, A ;

Odds, FC ;

Colebunders, R ;

Ieven, M ;

Goossens, H .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1997, 16 (04) :296-300

[8] Candida dubliniensis:: Characteristics and identification [J].

Sullivan, D ;

Coleman, D .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (02) :329-334

[9] CANDIDA DUBLINIENSIS SP-NOV - PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF A NOVEL SPECIES ASSOCIATED WITH ORAL CANDIDOSIS IN HIV-INFECTED INDIVIDUALS [J].

SULLIVAN, DJ ;

WESTERNENG, TJ ;

HAYNES, KA ;

BENNETT, DE ;

COLEMAN, DC .

MICROBIOLOGY-UK, 1995, 141 :1507-1521

[10] Rapid identification of Candida species by DNA fingerprinting with PCR [J].

Thanos, M ;

Schonian, G ;

Meyer, W ;

Schweynoch, C ;

Graser, Y ;

Mitchell, TG ;

Presber, W ;

Tietz, HJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (03) :615-621