Chemokine CXCL16, a scavenger receptor, induces proliferation and invasion of first-trimester human trophoblast cells in an autocrine manner

被引：71

作者：

Huang, Y ^{[1
]}

Zhu, XY ^{[1
]}

Du, MR ^{[1
]}

Wu, X ^{[1
]}

Wang, MY ^{[1
]}

Li, DJ ^{[1
]}

机构：

[1] Fudan Univ, Shanghai Med Coll, Hosp & Inst Obstet & Gynecol, Lab Reprod Immunol, Shanghai 200011, Peoples R China

来源：

HUMAN REPRODUCTION | 2006年 / 21卷 / 04期

关键词：

chemokine; chemokine receptor; invasion; proliferation; trophoblast cell;

D O I：

10.1093/humrep/dei436

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

BACKGROUND: The aim of this study was to investigate whether CXCL16/CXCR6, a newly identified chemokine pair, is expressed in first-trimester human placenta and whether they affect the trophoblast cell biology, since we have found CXCR6 highly transcribed in first-trimester human trophoblast cells previously. METHODS: We analysed the transcription and translation of CXCR6 and CXCL16 in purified first-trimester human trophoblast cells by real-time RT-PCR and immunochemical staining. We then examined the kinetic secretion of CXCL16 in the supernatant of primary-cultured trophoblast by enzyme-linked immunosorbent assay. We further investigated effects of CXCL16 on the proliferation and invasion of trophoblast cells in vitro. RESULTS: We found the chemokine pair CXCL16/CXCR6 was transcribed and translated in first-trimester trophoblast cells and JAR line. In addition, the primary-cultured trophoblasts secreted CXCL16 spontaneously and continuously in 100-h culture. Treating trophoblasts with CXCL16 induced marked proliferation and invasion in vitro. CONCLUSION: The findings from this study have demonstrated for the first time that CXCR6 and CXCL16 are co-expressed by first-trimester human trophoblast cells and stimulate their proliferation and invasion in an autocrine/paracrine manner. It suggests that CXCL16 plays important roles in human extravillous cytotrophoblast invasion and placentation.

引用

页码：1083 / 1091

页数：9

共 40 条

[1] The transmembrane CXC-chemokine ligand 16 is induced by IFN-γ and TNF-α and shed by the activity of the disintegrin-like metalloproteinase ADAM10 [J].