Nitric oxide synthase expression in human neuroblastoma cell line induced by cytokines

被引：15

作者：

Ogura, T

Esumi, H

机构：

[1] Investigative Treatment Division, Natl. Cancer Ctr. Research Institute, Kashiwa, Chiba, 277, East, 5-I

来源：

NEUROREPORT | 1996年 / 7卷 / 04期

关键词：

inducible nitric oxide synthase; neuroblastoma cell; human; cytokines; NADPH-diaphorase;

D O I：

10.1097/00001756-199603220-00003

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

THE human neuroblastoma cell line NB-39-nu expressed mRNA coding for inducible nitric oxide synthase (iNOS) following treatment with a combination of interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha). The level of iNOS mRNA peaked 24h after stimulation and had declined by about 25% after 48h. Trace levels of iNOS mRNA were detected after treatment with IFN-gamma alone, and its mRNA level was synergistically enhanced by simultaneous treatment with TNF-alpha. Neither bacterial lipopolysaccharide nor interleukin-1 beta (IL-1 beta) showed synergistic effects as great as that of TNF-alpha on iNOS gene expression. Dexamethasone inhibited the induction of iNOS mRNA by IFN-gamma and TNF-alpha. Induction of iNOS was confirmed by NADPH-diaphorase staining and by immunostaining with human iNOS-specific antibody.

引用

页码：853 / 856

页数：4

共 25 条

[1] COLCHICINE INDUCES APOPTOSIS IN CEREBELLAR GRANULE CELLS [J].