A three-megabase yeast artificial chromosome Contig spanning the C57BL mouse Igh locus

被引：36

作者：

Chevillard, C

Ozaki, J

Herring, CD

Riblet, R

机构：

[1] Torrey Pines Inst Mol Studies, San Diego, CA 92121 USA

[2] Fac Med Immunol & Genet Parasit Dis, INSERM, Marseille, France

[3] Vanderbilt Univ, Dept Med, Div Med Genet, Nashville, TN 37232 USA

[4] Univ Wisconsin, Dept Genet, Madison, WI 53706 USA

来源：

JOURNAL OF IMMUNOLOGY | 2002年 / 168卷 / 11期

关键词：

D O I：

10.4049/jimmunol.168.11.5659

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

The mouse Ig H chain (Igh) complex locus is composed of >100 gene segments encoding the variable, diversity, joining, and constant portions of the Ab H chain protein. To advance the characterization of this locus and to identify all the V-H genes, we have isolated the entire region from C57BL/6 and C57BL/10 as a yeast artificial chromosome contig. The mouse Igh locus extends approximately three megabases and contains at least 134 V H genes classified in 15 partially interspersed families. Two non-Igh pseudogenes (Odc-rs8 and Rpl32-rs14) were localized in the distal part of the locus. This physical yeast artificial chromosome map will provide important structure and guidance for the sequencing of this large, complex, and highly repetitive locus.

引用

页码：5659 / 5666

页数：8

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