A novel real-time PCR method for KIR genotyping

被引：26

作者：

Alves, L. G. T. ^{[1
]}

Rajalingam, R. ^{[2
]}

Canavez, F. ^{[1
]}

机构：

[1] Almeda Minist Rocha Azevedo, Genoa Biotechnol SA, Div Genet, BR-01410000 Sao Paulo, Brazil

[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pathol & Lab Med, Immunogenet Ctr, Los Angeles, CA 90095 USA

来源：

TISSUE ANTIGENS | 2009年 / 73卷 / 02期

关键词：

KIR genotyping; noninvasive sampling; real-time PCR; SYBR green; RECEPTOR KIR; DIVERSITY; DISEASE; GENES;

D O I：

10.1111/j.1399-0039.2008.01184.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Genes encoding killer cell immunoglobulin-like receptors (KIRs) are variable among individuals. Sequence-specific primer-directed polymerase chain reaction (PCR) amplification (PCR-SSP) and sequence-specific oligonucleotide hybridization of the PCR-amplified products (PCR-SSO) are the methods currently used to characterize the diversity of KIR gene content. Both these methods include time-consuming post-PCR analyses. Here, we developed a real-time PCR method that identifies the presence or absence of 16 KIR genes during PCR and avoids post-PCR analyses. This method is specific, sensitive, shortens the turnaround time compared with the conventional PCR-SSP and PCR-SSO methods, and it can be easily adapted for automation.

引用

页码：188 / 191

页数：4

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