A Rapid MALDI-TOF MS Identification Database at Genospecies Level for Clinical and Environmental Aeromonas Strains

被引:53
作者
Benagli, Cinzia [1 ]
Demarta, Antonella [1 ]
Caminada, AnnaPaola [1 ]
Ziegler, Dominik [2 ]
Petrini, Orlando [1 ]
Tonolla, Mauro [1 ,3 ]
机构
[1] Inst Microbiol, Bellinzona, Switzerland
[2] Mabritec AG, Riehen, Switzerland
[3] Univ Geneva, Dept Plant Biol, Microbiol Unit, Geneva, Switzerland
来源
PLOS ONE | 2012年 / 7卷 / 10期
关键词
DESORPTION/IONIZATION-MASS SPECTROMETRY; GENUS AEROMONAS; SP-NOV; PHYLOGENETIC ANALYSIS; SUBSP NOV; BIVALVE MOLLUSKS; SALMONICIDA; HYDROPHILA; SEQUENCES; GYRB;
D O I
10.1371/journal.pone.0048441
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The genus Aeromonas has undergone a number of taxonomic and nomenclature revisions over the past 20 years, and new (sub) species and biogroups are continuously described. Standard identification methods such as biochemical characterization have deficiencies and do not allow clarification of the taxonomic position. This report describes the development of a matrix-assisted laser desorption/ ionisation-time of flight mass spectrometry (MALDI-TOF MS) identification database for a rapid identification of clinical and environmental Aeromonas isolates.
引用
收藏
页数:6
相关论文
共 43 条
[1]  
Austin BaA, 1996, GENUS AEROMONAS, P197
[2]   Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for the Identification of Clinically Relevant Bacteria [J].
Benagli, Cinzia ;
Rossi, Viviana ;
Dolina, Marisa ;
Tonolla, Mauro ;
Petrini, Orlando .
PLOS ONE, 2011, 6 (01)
[3]   AEROMONAS-ENTEROPELOGENES AND AEROMONAS-ICHTHIOSMIA ARE IDENTICAL TO AEROMONAS-TROTA AND AEROMONAS-VERONII, RESPECTIVELY, AS REVEALED BY SMALL-SUBUNIT RIBOSOMAL-RNA SEQUENCE-ANALYSIS [J].
COLLINS, MD ;
MARTINEZMURCIA, AJ ;
CAI, J .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1993, 43 (04) :855-856
[4]   Polyphasic taxonomic study of "Aeromonas eucrenophila-like" isolates from clinical and environmental sources [J].
Demarta, A ;
Huys, G ;
Tonolla, M ;
Swings, J ;
Peduzzi, R .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2004, 27 (03) :343-349
[5]   Epidemiological relationships between Aeromonas strains isolated from symptomatic children and household environments as determined by ribotyping [J].
Demarta, A ;
Tonolla, M ;
Caminada, A ;
Beretta, M ;
Peduzzi, R .
EUROPEAN JOURNAL OF EPIDEMIOLOGY, 2000, 16 (05) :447-453
[6]   Differentiation of Aeromonas isolated from drinking water distribution systems using matrix-assisted laser desorption/ionization-mass spectrometry [J].
Donohue, Maura J. ;
Best, Jennifer M. ;
Smallwood, Anthony W. ;
Kostich, Mitchell ;
Rodgers, Mark ;
Shoemaker, Jody A. .
ANALYTICAL CHEMISTRY, 2007, 79 (05) :1939-1946
[7]   The development of a matrix-assisted laser desorption/ionization mass spectrometry-based method for the protein fingerprinting and identification of Aeromonas species using whole cells [J].
Donohue, Maura J. ;
Smallwood, Anthony W. ;
Pfaller, Stacy ;
Rodgers, Mark ;
Shoemaker, Jody A. .
JOURNAL OF MICROBIOLOGICAL METHODS, 2006, 65 (03) :380-389
[8]   Taxonomic study of sucrose-positive Aeromonas jandaei-like isolates from faeces, water and eels:: emendation of A-jandaei Carnahan et al. 1992 [J].
Esteve, C ;
Valera, L ;
Gutiérrez, C ;
Ventosa, A .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2003, 53 :1411-1419
[9]   Characterization of intact microorganisms by MALDI mass spectrometry [J].
Fenselau, C ;
Demirev, PA .
MASS SPECTROMETRY REVIEWS, 2001, 20 (04) :157-171
[10]   Rapid identification of Legionella spp. by MALDI-TOF MS based protein mass fingerprinting [J].
Gaia, Valeria ;
Casati, Simona ;
Tonolla, Mauro .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2011, 34 (01) :40-44