Characterization of intact microorganisms by MALDI mass spectrometry

被引：645

作者：

Fenselau, C ^{[1
]}

Demirev, PA ^{[1
]}

机构：

[1] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA

来源：

MASS SPECTROMETRY REVIEWS | 2001年 / 20卷 / 04期

关键词：

bacteria detection; biomarker identification; bioinformatics; spore characterization; virus characterization;

D O I：

10.1002/mas.10004

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The application of MALDI mass spectrometry to desorb protein biomarkers from intact viruses, bacteria, fungus, and spores is the focus of this review. Instrumentation, sample collection, sample preparation, and algorithms for data analysis are summarized. Optimally these analyses should be carried out in less than five minutes. Successful applications are discussed from biotechnology, cell biology, and the pharmaceutical industry (C) 2002 John Wiley Sons, Inc.

引用

页码：157 / 171

页数：15

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