Observation of tetrahydrofolylpolyglutamic acid in bacteria cells by matrix-assisted laser desorption/ionization mass spectrometry

被引：11

作者：

Arnold, RJ ^{[1
]}

Reilly, JP ^{[1
]}

机构：

[1] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2000年 / 281卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1006/abio.2000.4527

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tetrahydrofolylpolyglutamic acid in whole bacteria cells and cell lysates is analyzed by matrix-assisted laser desorption/ionization mass spectrometry. The speed, mass information, and tolerance to impurities of this technique make it ideal for monitoring the glutamation levels of folic acid in biological systems. Folylpolyglutamic acid is observed in a few strains of E. coli and two species of Staphylococcus bacteria. The effects of growth time, growth media, and the addition of methotrexate, a dihydrofolate reductase inhibitor, are also studied. (C) 2000 Academic Press.

引用

页码：45 / 54

页数：10

共 36 条

[1] [Anonymous], 1997, CURRENT PROTOCOLS MO
[2] Monitoring the growth of a bacteria culture by MALDI-MS of whole cells
Arnold, RJ
Karty, JA
Ellington, AD
Reilly, JP
[J]. ANALYTICAL CHEMISTRY, 1999, 71 (10) : 1990 - 1996
[3] Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry
Arnold, RJ
Reilly, JP
[J]. ANALYTICAL BIOCHEMISTRY, 1999, 269 (01) : 105 - 112
[4] Arnold RJ, 1998, RAPID COMMUN MASS SP, V12, P630, DOI 10.1002/(SICI)1097-0231(19980529)12:10<630::AID-RCM206>3.3.CO
[5] 2-S
[6] DIFFERENTIATION OF BACTERIA USING PROTEIN PROFILES FROM MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY
CAIN, TC
LUBMAN, DM
WEBER, WJ
[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1994, 8 (12) : 1026 - 1030
[7] The rapid identification of intact microorganisms using mass spectrometry
Claydon, MA
Davey, SN
EdwardsJones, V
Gordon, DB
[J]. NATURE BIOTECHNOLOGY, 1996, 14 (11) : 1584 - 1586
[8] Space-velocity correlation focusing
Colby, SM
Reilly, JP
[J]. ANALYTICAL CHEMISTRY, 1996, 68 (08) : 1419 - 1428
[9] Dai YQ, 1999, RAPID COMMUN MASS SP, V13, P73
[10] Monitoring protein expression in whole bacterial cells with MALDI time-of-flight mass spectrometry
Easterling, ML
Colangelo, CM
Scott, RA
Amster, IJ
[J]. ANALYTICAL CHEMISTRY, 1998, 70 (13) : 2704 - 2709

← 1 2 3 4 →