Bone regeneration on computer-designed nano-fibrous scaffolds

被引:163
作者
Chen, VJ
Smith, LA
Ma, PX
机构
[1] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Sch Dent, Dept Biol & Mat Sci, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Mol Sci & Engn Ctr, Ann Arbor, MI 48109 USA
关键词
polylactic acid; rapid prototyping; tissue engineering; 3-D printing; nano fiber; ostcoblast;
D O I
10.1016/j.biomaterials.2006.02.043
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The ability to control architectural features in tissue engineering scaffolds is critical to the success of neo-tissue regeneration. In this work, reverse solid freeform fabrication and thermal phase separation of poly((L)-lactic acid) (PLLA) solutions were used to create threedimensional nano-fibrous (NF) scaffolds with complex geometries on the macro- and micro-scales. This approach allows for the fabrication of NF matrices while having precise control of internal pore size and structure, as well as external scaffold shape including architectures generated from computed-tomography scans and histological sections. In vitro cell cultivation experiments with MC3T3-E1 preosteoblasts were performed on NF scaffolds and oil similarly designed solid-walled (SW) scaffolds that did not have nano-fibers. Proliferation studies showed significantly more cells on NF scaffolds after 7 d. In differentiation studies, the NF scaffolds displayed more uniform matrix and mineral production throughout. Real-time PCR also showed significantly higher expression of osteocalcin and bone sialoprotein mRNAs after 2 and 6 weeks in the NF scaffolds. Expression of type I collagen mRNA was lower ill NF scaffolds which possibly indicates quicker differentiation oil the NF substrate. In summary, we controlled the geometry of NF PLLA scaffolds at multiple size scales, and the in vitro results showed that these NF scaffolds were advantageous to control scaffolds for bone tissue engineering. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3973 / 3979
页数:7
相关论文
共 25 条
[1]  
Abrams GA, 2000, CELL TISSUE RES, V299, P39, DOI 10.1007/s004410050004
[2]   DEDIFFERENTIATED CHONDROCYTES REEXPRESS THE DIFFERENTIATED COLLAGEN PHENOTYPE WHEN CULTURED IN AGAROSE GELS [J].
BENYA, PD ;
SHAFFER, JD .
CELL, 1982, 30 (01) :215-224
[3]   Nano-fibrous poly(L-lactic acid) scaffolds with interconnected spherical macropores [J].
Chen, VJ ;
Ma, PX .
BIOMATERIALS, 2004, 25 (11) :2065-2073
[4]   Effects of synthetic micro- and nano-structured surfaces on cell behavior [J].
Flemming, RG ;
Murphy, CJ ;
Abrams, GA ;
Goodman, SL ;
Nealey, PF .
BIOMATERIALS, 1999, 20 (06) :573-588
[5]   Forces on adhesive contacts affect cell function [J].
Galbraith, CG ;
Sheetz, MP .
CURRENT OPINION IN CELL BIOLOGY, 1998, 10 (05) :566-571
[6]   Tissue engineering - Current challenges and expanding opportunities [J].
Griffith, LG ;
Naughton, G .
SCIENCE, 2002, 295 (5557) :1009-+
[7]   BONE-MATRIX RGD GLYCOPROTEINS - IMMUNOLOCALIZATION AND INTERACTION WITH HUMAN PRIMARY OSTEOBLASTIC BONE-CELLS IN-VITRO [J].
GRZESIK, WJ ;
ROBEY, PG .
JOURNAL OF BONE AND MINERAL RESEARCH, 1994, 9 (04) :487-496
[8]   Self-assembly and mineralization of peptide-amphiphile nanofibers [J].
Hartgerink, JD ;
Beniash, E ;
Stupp, SI .
SCIENCE, 2001, 294 (5547) :1684-1688
[9]   Extensive neurite outgrowth and active synapse formation on self-assembling peptide scaffolds [J].
Holmes, TC ;
de Lacalle, S ;
Su, X ;
Liu, GS ;
Rich, A ;
Zhang, SG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6728-6733
[10]   Scaffold design and fabrication technologies for engineering tissues - state of the art and future perspectives [J].
Hutmacher, DW .
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2001, 12 (01) :107-124