Enzyme assisted extraction of polysaccharides from the fruit of Cornus officinalis

被引:143
作者
You, Qinghong [1 ,2 ,3 ]
Yin, Xiulian [1 ,2 ,4 ]
Zhao, Yuping [1 ]
机构
[1] Huaiyin Inst Technol, Sch Life Sci & Chem Engn, Huaian 223001, Peoples R China
[2] Huaiyin Inst Technol, Jiangsu Prov Engn Lab Biomass Convers & Proc Inte, Huaian 223001, Peoples R China
[3] Jiangsu Key Lab Biomass Based Energy & Enzyme Tec, Huaian 223300, Peoples R China
[4] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China
关键词
Enzyme assisted extraction; Cornus officinalis; Response surface methodology; The yield of Cornus officinalis polysaccharides; RESPONSE-SURFACE METHODOLOGY; OPTIMIZATION;
D O I
10.1016/j.carbpol.2013.06.036
中图分类号
O69 [应用化学];
学科分类号
070301 [无机化学];
摘要
Process of enzyme assisted extraction (EAE) of polysaccharides from Cornus officinalis was optimized by response surface methodology (RSM). The influence of four different factors on the yield of C. officinalis polysaccharides (COP) was studied. Results showed that the optimal conditions were compound enzyme amount of 2.15%, extraction pH of 4.2, extraction temperature of 55 degrees C and extraction time of 97 min. Under these conditions, the COP yield was 9.29 +/- 0.31%, which was well in agreement with the value predicted by the model. The three methods, EAE, hot water extraction (HWE), ultrasound-assisted extraction (UAE) for extracting COP by RSM were further compared. Results showed that EAE had the largest yield of polysaccharides with lower equipment cost. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:607 / 610
页数:4
相关论文
共 9 条
[1]
Cai-ying Z., 2007, FOOD SCI, V28, P105
[2]
Cai-ying Z., 2007, LISHIZHEN MED MAT ME, V18, P313
[3]
Physical characteristics and antioxidant effect of polysaccharides extracted by boiling water and enzymolysis from Grifola frondosa [J].
Fan, Yina ;
Wu, Xiangyang ;
Zhang, Min ;
Zhao, Ting ;
Zhou, Ye ;
Han, Liang ;
Yang, Liuqing .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2011, 48 (05) :798-803
[4]
Extraction of antioxidant pectic-polysaccharide from mangosteen (Garcinia mangostana) rind Optimization using response surface methodology [J].
Gan, Chee-Yuen ;
Latiff, Aishah A. .
CARBOHYDRATE POLYMERS, 2011, 83 (02) :600-607
[5]
Optimization of alcohol insoluble polysaccharides (AIPS) extraction from the Parkia speciosa pod using response surface methodology (RSM) [J].
Gan, Chee-Yuen ;
Manaf, Normaliza Hj. Abdul ;
Latiff, Aishah A. .
CARBOHYDRATE POLYMERS, 2010, 79 (04) :825-831
[6]
Carbohydrate analysis by a phenol-sulfuric acid method in microplate format [J].
Masuko, T ;
Minami, A ;
Iwasaki, N ;
Majima, T ;
Nishimura, SI ;
Lee, YC .
ANALYTICAL BIOCHEMISTRY, 2005, 339 (01) :69-72
[7]
Optimization of enzyme assisted extraction of polysaccharides from Tricholoma matsutake by response surface methodology [J].
Yin, Xiulian ;
You, Qinghong ;
Jiang, Zhonghai .
CARBOHYDRATE POLYMERS, 2011, 86 (03) :1358-1364
[8]
Yunxiang Y., 2009, J FOOD SCI BIOTECHNO, V28, P764
[9]
Optimization of enzyme-assisted extraction of the Lycium barbarum polysaccharides using response surface methodology [J].
Zhang, Jiao ;
Jia, Shaoyi ;
Liu, Yong ;
Wu, Songhai ;
Ran, Jingyu .
CARBOHYDRATE POLYMERS, 2011, 86 (02) :1089-1092