Small-interference RNA-mediated knock-down of aldehyde oxidase 1 in 3T3-L1 cells impairs adipogenesis and adiponectin release

被引：49

作者：

Weigert, Johanna ^{[1
]}

Neumeier, Markus ^{[1
]}

Bauer, Sabrina ^{[1
]}

Mages, Wolfgang ^{[2
]}

Schnitzbauer, Andreas A. ^{[3
]}

Obed, Aiman ^{[3
]}

Groeschl, Benedikt ^{[1
]}

Hartmann, Arndt ^{[4
]}

Schaeffler, Andreas ^{[1
]}

Aslanidis, Charalampos ^{[5
]}

Schoelmerich, Juergen ^{[1
]}

Buechler, Christa ^{[1
]}

机构：

[1] Regensburg Univ Hosp, Dept Internal Med 1, D-93042 Regensburg, Germany

[2] Univ Regensburg, Inst Genet, D-93053 Regensburg, Germany

[3] Regensburg Univ Hosp, Dept Surg, D-93042 Regensburg, Germany

[4] Regensburg Univ Hosp, Inst Pathol & Mol Pathol, D-93042 Regensburg, Germany

[5] Regensburg Univ Hosp, Inst Clin Chem & Lab Med, D-93042 Regensburg, Germany

来源：

FEBS LETTERS | 2008年 / 582卷 / 19期

关键词：

adipocytes; adipose tissue; peroxisome proliferator-activated receptor agonist;

D O I：

10.1016/j.febslet.2008.07.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aldehyde oxidase 1 (AOX1) is highly abundant in the liver and oxidizes aldehydes thereby generating reactive oxygen species. Enzymes involved in detoxification of aldehydes are expressed in adipocytes and alter adipogenesis, therefore the functional role of AOX1 in adipocytes was analyzed. AOX1 mRNA was higher in visceral compared to subcutaneous human adipose tissue but AOX1 protein was detected in both fat depots. AOX1 expression in adipocytes was confirmed by immunohistochemistry and immunoblot. AOX1 was induced during adipocytic differentiation and was downregulated by fenofibrate in differentiated cells. Knock-down of AOX1 in preadipocytes led to impaired lipid storage and adiponectin release in the differentiated cells. These data indicate that AOX1 is essential for adipogenesis and may link energy and drug metabolism. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：2965 / 2972

页数：8

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