Biocompatible, nanogold-particle fluorescence enhancer for fluorophore mediated, optical immunosensor

被引：72

作者：

Hong, B ^{[1
]}

Kang, KA ^{[1
]}

机构：

[1] Univ Louisville, Dept Chem Engn, Louisville, KY 40292 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2006年 / 21卷 / 07期

基金：

美国国家科学基金会;

关键词：

fluorescence enhancement; nanogold particle; biocompatible solvent; biosensor; cardiac markers;

D O I：

10.1016/j.bios.2005.04.007

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Fluorophores have been used as effective signal mediators for detecting biomarkers in biosamples. The enhancement of the fluorescence can, therefore, improve the sensitivity of fluorophore-mediated biosensors. A nanogold particle (NGP), when placed at an appropriate distance from a fluorophore, can effectively enhance the fluorescence by transferring the free electrons of the fluorophore, normally used for self-quenching, to the strong surface plasmon polariton field (SPPF) of the NGP. We found that some organic solvents can also enhance the fluorescence significantly. To maximize the fluorescence enhancement, novel, biocompatible nanogold particle reagents (NGPRs) were developed by combining NGPs and biocompatible solvents and tested. The level of enhancement by NGPRs was found to be additively contributed by two enhancers. These NGPRs were able to increase the signal of a fiber-optic biosensor as much as 10 times and accurately quantify some of the important cardiac markers at a tens of picomolar level. These novel enhancers are expected to be effective for fluorophore-mediated bioimaging as well as biosensing. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：1333 / 1338

页数：6

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