Gelatin-Methacrylamide Hydrogels as Potential Biomaterials for Fabrication of Tissue-Engineered Cartilage Constructs

被引：717

作者：

Schuurman, Wouter ^{[1
,2
]}

Levett, Peter A. ^{[3
]}

Pot, Michiel W. ^{[1
,4
]}

van Weeren, Paul Rene ^{[2
]}

Dhert, Wouter J. A. ^{[1
,5
]}

Hutmacher, Dietmar W. ^{[3
]}

Melchels, Ferry P. W. ^{[1
,3
]}

Klein, Travis J. ^{[3
]}

Malda, Jos ^{[1
,3
]}

机构：

[1] Univ Med Ctr, Dept Orthopaed, Utrecht, Netherlands

[2] Univ Utrecht, Dept Equine Sci, Fac Vet Med, NL-3508 TC Utrecht, Netherlands

[3] Queensland Univ Technol, Inst Hlth & Biomed Innovat, Kelvin Grove 4059, Australia

[4] Univ Twente, MIRA Inst Biomed Technol & Tech Med, NL-7500 AE Enschede, Netherlands

[5] Univ Utrecht, Fac Vet Med, NL-3508 TC Utrecht, Netherlands

来源：

MACROMOLECULAR BIOSCIENCE | 2013年 / 13卷 / 05期

基金：

澳大利亚研究理事会;

关键词：

additive manufacturing; cartilage; gelatin; hydrogels; tissue engineering; POLY(ETHYLENE GLYCOL) HYDROGELS; HYALURONIC-ACID HYDROGELS; ARTICULAR CHONDROCYTES; MECHANICAL-PROPERTIES; ECM PRODUCTION; CROSS-LINKING; STEM-CELLS; I COLLAGEN; THICKNESS; DIFFERENTIATION;

D O I：

10.1002/mabi.201200471

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness and swelling properties. GelMA solutions have a low viscosity at 37 degrees C, which is incompatible with most biofabrication approaches. However, incorporation of hyaluronic acid (HA) and/or co-deposition with thermoplastics allows gelMA to be used in biofabrication processes. These attributes may allow engineered constructs to match the natural functional variations in cartilage mechanical and geometrical properties.

引用

页码：551 / 561

页数：11

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