Real-time assays for the assembly-disassembly cycle of COP coats on liposomes of defined size

被引：25

作者：

Bigay, J ^{[1
]}

Antonny, B ^{[1
]}

机构：

[1] CNRS, Inst Pharmacol Mol & Cellulaire, Valbonne, France

来源：

GTPASES REGULATING MEMBRANE DYNAMICS | 2005年 / 404卷

关键词：

D O I：

10.1016/S0076-6879(05)04010-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The assembly-disassembly cycle of COPI and COPII coats is controlled by the GTPase cycle of the small G proteins Arf1 and Sar. We describe here two spectroscopic assays that enable real-time studies of some elementary steps of coat assembly and disassembly on artificial liposomes of defined composition and curvature. A flotation assay to assess the effect of membrane curvature on protein adsorption to liposomes is also presented.

引用

页码：95 / 107

页数：13

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