High throughput sequencing reveals the diversity of TRB-CDR3 repertoire in patients with psoriasis vulgaris

被引：16

作者：

Cao, Xiaofang ^{[1
]}

Wa, Qingbiao ^{[2
]}

Wang, Qidi ^{[1
]}

Li, Lin ^{[2
]}

Liu, Xin ^{[1
,3
]}

An, Lisha ^{[1
]}

Cai, Ruikun ^{[1
]}

Du, Meng ^{[1
,4
]}

Qiu, Yue ^{[1
,4
]}

Han, Jian ^{[5
]}

Wang, Chunlin ^{[1
,6
]}

Wang, Xingyu ^{[1
,3
]}

Guo, Changlong ^{[1
]}

Lu, Yonghong ^{[2
]}

Ma, Xu ^{[1
,4
]}

机构：

[1] Natl Res Inst Family Planning, Dept Genet, Beijing 100081, Peoples R China

[2] Chengdu Second Peoples Hosp, Dept Dermatovenereol, Chengdu 610017, Peoples R China

[3] Beijing Hypertens League Inst, Lab Human Genet, Beijing 100043, Peoples R China

[4] Peking Union Med Coll, Grad Sch, Beijing 100730, Peoples R China

[5] HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA

[6] Stanford Univ, Stanford Genome Technol Ctr, Palo Alto, CA 94003 USA

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2016年 / 40卷

基金：

对外科技合作项目（国际科技项目）; 中国国家自然科学基金;

关键词：

Psoriasis; High-throughput sequencing; TRB-CDR3; repertoire; T-CELLS; SKIN; IMMUNOPATHOGENESIS; IMMUNOGENETICS; PATHOGENESIS; TONSILS; LESIONS;

D O I：

10.1016/j.intimp.2016.10.004

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Psoriasis is a T cell-mediated chronic inflammatory skin disease with inflammatory cell infiltrates in the dermis and epidermis. Previous studies suggested that there are some expanded T-cell receptor (TCR) clones in psoriatic skin. However, the effect of psoriasis on the immunological characteristics of TCR in circulating blood has not been reported. To address this, we performed high-throughput sequencing to reveal the immunological characteristics of TCR beta chain (TRB) in both psoriasis patients and healthy controls. Our results revealed that the TRBCDR3 region of psoriasis patients had distinctive immunological characteristics compared with that of healthy controls, including V gene usage, nt of N addition. In addition, three types of TRB-CDR3 peptides were found highly relevant to psoriasis. Our findings show the comprehensive characteristics of psoriasis on the TRB-CDR3 repertoire of circulating blood at sequence-level resolution. These findings may contribute to a better understanding of the pathogenesis of psoriasis and open opportunities to explore potential therapeutic targets. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：487 / 491

页数：5

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