Combined microscale mechanical topography and chemical patterns on polymer cell culture substrates

被引:170
作者
Charest, JL [1 ]
Eliason, MT [1 ]
García, AJ [1 ]
King, WP [1 ]
机构
[1] Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA
关键词
micropatterning; surface topography; chemical patterns; alignment; adhesion;
D O I
10.1016/j.biomaterials.2005.11.022
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
This paper presents a technique to independently form mechanical topography and surface chemical patterns oil polymer cell Substrates. and studies the response of osteoblast cells to these surface patterns. The patterns were formed in two separate steps: hot embossing imprint lithography formed the mechanical topography and microcontact printing created the chemical pattern. The resulting substrate had surface features consisting of embossed grooves 4 mu m deep and 8 mu m wide spaced by 16 mu m wide mesas and microcontact printed adhesive lanes 10 mu m wide with spacings that ranged from 10 to 100 mu m. When presented with either mechanical topography or chemical patterns alone, the cells significantly aligned to the pattern presented. When presented with mechanical topography overlaid with an orthogonal chemical pattern, the cells aligned to the mechanical topography. As the chemical pattern spacing was increased, osteoblasts remained aligned to the mechanical topography. Unlike traditional microfabrication approaches based on photolithography and wet chemistry, the patterning technique presented is compatible with a large number of biomaterials, Could form patterns with features much smaller than 1 mu m, and is highly scalable to large substrates. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2487 / 2494
页数:8
相关论文
共 56 条
[1]  
Anderson CW, 2003, ADV FIN ECO, V8, P1
[2]   Submicron-scale topographical control of cell growth using holographic surface relief grating [J].
Baac, HW ;
Lee, JH ;
Seo, JM ;
Park, TH ;
Chung, H ;
Lee, SD ;
Kim, SJ .
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 2004, 24 (1-2) :209-212
[3]  
Bhatia SN, 1997, J BIOMED MATER RES, V34, P189, DOI 10.1002/(SICI)1097-4636(199702)34:2<189::AID-JBM8>3.0.CO
[4]  
2-M
[5]   Synergistic and hierarchical adhesive and topographic guidance of BHK cells [J].
Britland, S ;
Morgan, H ;
WojiakStodart, B ;
Riehle, M ;
Curtis, A ;
Wilkinson, C .
EXPERIMENTAL CELL RESEARCH, 1996, 228 (02) :313-325
[7]   Biomedical surface science: Foundations to frontiers [J].
Castner, DG ;
Ratner, BD .
SURFACE SCIENCE, 2002, 500 (1-3) :28-60
[8]   Hot embossing for micropatterned cell substrates [J].
Charest, JL ;
Bryant, LE ;
Garcia, AJ ;
King, WP .
BIOMATERIALS, 2004, 25 (19) :4767-4775
[9]   Micropatterned surfaces for control of cell shape, position, and function [J].
Chen, CS ;
Mrksich, M ;
Huang, S ;
Whitesides, GM ;
Ingber, DE .
BIOTECHNOLOGY PROGRESS, 1998, 14 (03) :356-363
[10]   Imprint lithography with 25-nanometer resolution [J].
Chou, SY ;
Krauss, PR ;
Renstrom, PJ .
SCIENCE, 1996, 272 (5258) :85-87