Apoptotic cell death and caspase 3 (CPP32) activation induced by calcium ionophore at low concentrations and their prevention by nerve growth factor in PC12 cells

被引：43

作者：

Takadera, T

Ohyashiki, T

机构：

[1] Department of Clinical Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa

[2] Department of Clinical Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1997年 / 249卷 / 01期

关键词：

calcium; apoptosis; nerve-growth factor; caspase-3 (CPP32); PC12; cells;

D O I：

10.1111/j.1432-1033.1997.00008.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A23187 (a calcium ionophore) at law concentration (0.1 mu M) induced apoptotic cell death (chromatin condensation and DNA fragmentation) accompanied by the activation of caspase-3 (CPP32), a member of the interleukin-1 beta-converting enzyme protease. On the other hand, A23187 at high concentration (2 mu M) induced neurotic cell death not accompanied by the activation of CPP32, Nerve growth factor inhibited the cell death and CPP32 activation induced by 0.1 mu M A23187, but not the fell death induced by 2 mu M A23187, Acylaspartyl-glutamyl-valyl-aspartyl-aldehyde, an inhibitor of CPP32. reduced the cell death induced by 0.1 mu M A23187. These results suggest that calcium-ion-induced apoptotic cell death was mediated by CPP32 activation in PC12 cells.

引用

页码：8 / 12

页数：5

共 29 条

[1] APOPTOSIS AND NECROSIS - 2 DISTINCT EVENTS INDUCED, RESPECTIVELY, BY MILD AND INTENSE INSULTS WITH N-METHYL-D-ASPARTATE OR NITRIC-OXIDE SUPEROXIDE IN CORTICAL CELL-CULTURES [J].