The application of CdTe@SiO2 particles in immunoassay

被引：34

作者：

Jin, Li ^{[1
]}

Yu, Dong-Dong ^{[2
]}

Liu, Yan ^{[1
]}

Zhao, Xiao-Li ^{[1
]}

Zhou, Jian-Guang ^{[1
,3
]}

机构：

[1] Jilin Univ, Coll Chem, Dept Analyt Chem, Changchun 130023, Peoples R China

[2] Jilin Univ, Hosp 1, Dept Infect Dis, Changchun 130021, Peoples R China

[3] Zhejiang Univ, Res Ctr Analyt Instrumentat, Hangzhou, Zhejiang, Peoples R China

来源：

TALANTA | 2008年 / 76卷 / 05期

关键词：

CdTe@SiO2 fluorescent particles; water-in-oil (W/O) emulsion; fluoroimmunoassay;

D O I：

10.1016/j.talanta.2008.05.002

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

CdTe@SiO2 fluorescent particles were synthesized via hydrolysis and condensation of tetraethyl orthosilicate (TEOS) in water-in-oil (W/O) emulsion. Uniform luminophore-doped silica nanoparticles with 100 nm in diameter were obtained using microemulsion method and characterized by SEM. Antibody proteins were successfully conjugated to the fluorescent particles by the reaction of avidin and biotin, which were confirmed by fluorescence spectra. CdTe@SiO2 fluorescent particles were potentially useful for the applications in biolabeling and imaging. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1053 / 1057

页数：5

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