Blood oxygen transport in rats under hypothermia combined with modification of the L-arginine-NO pathway

被引:16
作者
Zinchuk, VV [1 ]
Dorokhina, LV [1 ]
机构
[1] Grodno Med Univ, Dept Physiol, Grodno 230015, BELARUS
来源
NITRIC OXIDE-BIOLOGY AND CHEMISTRY | 2002年 / 6卷 / 01期
关键词
hemoglobin-oxygen affinity; blood; nitric oxide; NO donors; inhibitors of NO synthase; hypothermia; rat;
D O I
10.1006/niox.2001.0377
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nitric oxide (NO) has high affinity to heme and by interaction with oxyhemoglobin (HbO(2)) is converted into nitrate to form methemoglobin (MetHb) as a side product. In combining with deoxy-Hb NO yields a stable molecule of nitrosyl-hemoglobin (HbFe(II)NO) that can further be converted into nitrate and hemoglobin (Hb). In addition, Hb was shown to transport NO in a form of S-nitrosohemoglobin (SNO-Hb). These features of the Hb and NO interaction are important for blood oxygen transport including hemoglobin-oxygen affinity (HOA). The present investigation was aimed to study the blood oxygen transport indices (pO(2), pCO(2), pH, HOA, etc.) in rats under hypothermia combined with a modification Of L-arginine-NO pathway. To modify the L-arginine-NO pathway, rats were administered with A-nitro-L-arginine methyl ester (L-NAME), L-arginine, or sodium nitroprusside (SNP) intravenously before cooling. A substantial impairment of oxygen delivery and development of hypoxia, with an important contribution of HOA into the latter accompanied the deep hypothermia in rats. All the experimental groups developed metabolic acidosis, less pronounced in rats treated with L-arginine only. In the experiments with a modification of the L-arginine-NO pathway, an enhanced cold resistance, attenuated oxygen deficiency, and a weaker oxyhemoglobin dissociation curve (ODC) shift leftwards were observed only after the administration of L-arginine. Neither SNP nor L-NAME had not any protective effects. L-Arginine lowered the value of standard P-50 (pO(2), corresponding to 50% Hb saturation with oxygen at 37degreesC, pH 7.4, and pCO(2) = 40 mmHg). The actual P-50 (at actual pH, pCO(2) and temperature) decreased by approximately 15 mmHg and was significantly higher than that under hypothermia without the drug treatment (21.03 +/- 0.35 vs 17.45 +/- 0.60 mmHg). NO also can contribute to this system through different mechanisms (HOA modification, vascular tone regulation, peroxynitrite formation, and effects). (C) 2001 Elsevier Science (USA).
引用
收藏
页码:29 / 34
页数:6
相关论文
共 26 条
[1]   Peroxynitrite-mediated heme oxidation and protein modification of native and chemically modified hemoglobins [J].
Alayash, AI ;
Ryan, BAB ;
Cashon, RE .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1998, 349 (01) :65-73
[2]  
Amrani M, 1997, CIRCULATION, V96, P274
[3]   Effect of nitric oxide synthase inhibition on hypercapnia-induced hypothermia and hyperventilation [J].
Barros, RCH ;
Branco, LGS .
JOURNAL OF APPLIED PHYSIOLOGY, 1998, 85 (03) :967-972
[4]   S-Nitrosohemoglobin in the fetal circulation may represent a cycle for blood pressure regulation [J].
Funai, EF ;
Davidson, A ;
Seligman, SP ;
Finlay, TH .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 239 (03) :875-877
[5]   Low concentrations of nitric oxide increase oxygen affinity of sickle erythrocytes in vitro and in vivo [J].
Head, CA ;
Brugnara, C ;
MartinezRuiz, R ;
Kacmarek, RM ;
Bridges, KR ;
Kuter, D ;
Bloch, KD ;
Zapol, WM .
JOURNAL OF CLINICAL INVESTIGATION, 1997, 100 (05) :1193-1198
[6]   ENDOTHELIUM-DERIVED RELAXING FACTOR PRODUCED AND RELEASED FROM ARTERY AND VEIN IS NITRIC-OXIDE [J].
IGNARRO, LJ ;
BUGA, GM ;
WOOD, KS ;
BYRNS, RE ;
CHAUDHURI, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (24) :9265-9269
[7]   Dynamic state of S-nitrosothiols in human plasma and whole blood [J].
Jourd'heuil, D ;
Hallén, K ;
Feelisch, M ;
Grisham, MB .
FREE RADICAL BIOLOGY AND MEDICINE, 2000, 28 (03) :409-417
[8]   Physiological role of nitric oxide as an enhancer of oxygen transfer from erythrocytes to tissues [J].
Kosaka, H ;
Seiyama, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 218 (03) :749-752
[9]   HYPOXIA INDUCES ENDOTHELIN GENE-EXPRESSION AND SECRETION IN CULTURED HUMAN ENDOTHELIUM [J].
KOUREMBANAS, S ;
MARSDEN, PA ;
MCQUILLAN, LP ;
FALLER, DV .
JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (03) :1054-1057
[10]   Hypothermia suppresses nitric oxide elevation during reperfusion after focal cerebral ischemia in rats [J].
Kumura, E ;
Yoshimine, T ;
Takaoka, M ;
Hayakawa, T ;
Shiga, T ;
Kosaka, H .
NEUROSCIENCE LETTERS, 1996, 220 (01) :45-48