Genetic Modifier Screens Reveal New Components that Interact with the Drosophila Dystroglycan-Dystrophin Complex

被引：49

作者：

Kucherenko, Mariya M. ^{[1
,2
]}

Pantoja, Mario ^{[1
]}

Yatsenko, Andriy S. ^{[1
,2
]}

Shcherbata, Halyna R. ^{[1
]}

Fischer, Karin A. ^{[1
]}

Maksymiv, Dariya V. ^{[1
,2
]}

Chernyk, Yaroslava I. ^{[1
,2
]}

Ruohola-Baker, Hannele ^{[1
]}

机构：

[1] Univ Washington, Dept Biochem, Inst Stem Cell & Regenerat Med, Seattle, WA 98195 USA

[2] Ivan Franko Natl Univ Lviv, Dept Genet & Biotechnol, Lvov, Ukraine

来源：

PLOS ONE | 2008年 / 3卷 / 06期

关键词：

D O I：

10.1371/journal.pone.0002418

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Dystroglycan-Dystrophin (Dg-Dys) complex has a capacity to transmit information from the extracellular matrix to the cytoskeleton inside the cell. It is proposed that this interaction is under tight regulation; however the signaling/regulatory components of Dg-Dys complex remain elusive. Understanding the regulation of the complex is critical since defects in this complex cause muscular dystrophy in humans. To reveal new regulators of the Dg-Dys complex, we used a model organism Drosophila melanogaster and performed genetic interaction screens to identify modifiers of Dg and Dys mutants in Drosophila wing veins. These mutant screens revealed that the Dg-Dys complex interacts with genes involved in muscle function and components of Notch, TGF-beta and EGFR signaling pathways. In addition, components of pathways that are required for cellular and/or axonal migration through cytoskeletal regulation, such as Semaphorin-Plexin, Frazzled-Netrin and Slit-Robo pathways show interactions with Dys and/or Dg. These data suggest that the Dg-Dys complex and the other pathways regulating extracellular information transfer to the cytoskeletal dynamics are more intercalated than previously thought.

引用

页数：14

共 62 条

[21] The cytoplasmic dynein and kinesin motors have interdependent roles in patterning the Drosophila oocyte [J].

Duncan, JE ;

Warrior, R .

CURRENT BIOLOGY, 2002, 12 (23) :1982-1991

[22] Akt2 negatively regulates assembly of the POSH-MLK-JNK signaling complex [J].

Figueroa, C ;

Tarras, S ;

Taylor, J ;

Vojtek, AB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (48) :47922-47927

[23] Robo and Frazzled/DCC mediate dendritic guidance at the CNS midline [J].

Furrer, MP ;

Kim, S ;

Wolf, B ;

Chiba, A .

NATURE NEUROSCIENCE, 2003, 6 (03) :223-230

[24]

Guichard A, 1999, DEVELOPMENT, V126, P2663

[25] Role of dystrophin and utrophin for assembly and function of the dystrophin glycoprotein complex in non-muscle tissue [J].

Haenggi, T. ;

Fritschy, J. -M. .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2006, 63 (14) :1614-1631

[26] Polar transport in the Drosophilia oocyte requires Dynein and Kinesin I cooperation [J].

Januschke, J ;

Gervais, L ;

Dass, S ;

Kaltschmidt, JA ;

Lopez-Schier, H ;

St Johnston, D ;

Brand, AH ;

Roth, S ;

Guichet, A .

CURRENT BIOLOGY, 2002, 12 (23) :1971-1981

[27] Wing vein formation in Drosophila melanogaster:: Hairless is involved in the cross-talk between Notch and EGF signaling pathways [J].

Johannes, B ;

Preiss, A .

MECHANISMS OF DEVELOPMENT, 2002, 115 (1-2) :3-14

[28] Characterization of RACK1 function in Drosophila development [J].

Kadrmas, Julie L. ;

Smith, Mark A. ;

Pronovost, Stephen M. ;

Beckerle, Mary C. .

DEVELOPMENTAL DYNAMICS, 2007, 236 (08) :2207-2215

[29] Muscle and neuronal nicotinic acetylcholine receptors - Structure, function and pathogenicity [J].

Kalamida, Dimitra ;

Poulas, Konstantinos ;

Avramopoulou, Vassiliki ;

Fostieri, Efrosini ;

Lagoumintzis, George ;

Lazaridis, Konstantincis ;

Sideri, Anastasia ;

Zouridakis, Marios ;

Tzartos, Socrates J. .

FEBS JOURNAL, 2007, 274 (15) :3799-3845

[30] Touch and go: guidance cues signal to the growth cone cytoskelleton [J].

Kalil, K ;

Dent, EW .

CURRENT OPINION IN NEUROBIOLOGY, 2005, 15 (05) :521-526

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