Abnormal chloroplast development and growth inhibition in rice thioredoxin m knock-down plants

被引：51

作者：

Chi, Yong Hun ^{[1
,2
]}

Moon, Jeong Chan ^{[1
,2
]}

Park, Jin Ho ^{[1
,2
]}

Kim, Ho-Seung ^{[3
]}

Zulfugarov, Ismayil S. ^{[3
]}

Fanata, Wahyu Indra ^{[1
,2
]}

Jang, Ho Hee ^{[1
,2
]}

Lee, Jung Ro ^{[1
,2
]}

Lee, Young Mee ^{[1
,2
]}

Kim, Sun Tae ^{[1
,2
]}

Chung, Yong-Yoon ^{[4
]}

Lim, Chae Oh ^{[1
,2
]}

Kim, Jae-Yean ^{[1
,2
]}

Yun, Dae-Jin ^{[1
,2
]}

Lee, Choon-Hwan ^{[3
]}

Lee, Kyun Oh ^{[1
,2
]}

Lee, Sang Yeol ^{[1
,2
]}

机构：

[1] Gyeongsang Natl Univ, Div Appl Life Sci, EB NCRC, BK21 Program, Jinju 660701, South Korea

[2] Gyeongsang Natl Univ, Div Appl Life Sci, Plant Mol Biol & Biotechnol Res Ctr, BK21 Program, Jinju 660701, South Korea

[3] Pusan Natl Univ, Dept Mol Biol, Pusan 609735, South Korea

[4] Korea Univ, Sch Life Sci & Biotechnol, Seoul 136701, South Korea

来源：

PLANT PHYSIOLOGY | 2008年 / 148卷 / 02期

关键词：

D O I：

10.1104/pp.108.123547

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plant cells contain several thioredoxin isoforms that are characterized by subcellular localization and substrate specificity. Here, we describe the functional characterization of a rice (Oryza sativa) thioredoxin m isoform (Ostrxm) using a reverse genetics technique. Ostrxm showed green tissue-specific and light-responsive mRNA expression. Ostrxm was localized in chloroplasts of rice mesophyll cells, and the recombinant protein showed dithiothreitol-dependent insulin beta-chain reduction activity in vitro. RNA interference (RNAi) of Ostrxm resulted in rice plants with developmental defects, including semidwarfism, pale-green leaves, abnormal chloroplast structure, and reduced carotenoid and chlorophyll content. Ostrxm RNAi plants showed remarkably decreased F-v/F-m values under high irradiance conditions (1,000 mu mol m(-2) s(-1)) with delayed recovery. Two-dimensional electrophoresis and matrix-assisted laser-desorption/ionization time-of-flight analysis showed that the levels of several chloroplast proteins critical for photosynthesis and biogenesis were significantly decreased in Ostrxm RNAi plants. Furthermore, 2-Cys peroxiredoxin, a known target of thioredoxin, was present in oxidized forms, and hydrogen peroxide levels were increased in Ostrxm RNAi plants. The pleiotropic effects of Ostrxm RNAi suggest that Ostrxm plays an important role in the redox regulation of chloroplast target proteins involved in diverse physiological functions.

引用

页码：808 / 817

页数：10

共 43 条

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