The origin of the sodium-dependent NADH oxidation by the respiratory chain of Klebsiella pneumoniae

被引：51

作者：

Bertsova, YV ^{[1
]}

Bogachev, AV ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, AN Belozersky Inst Physicochem Biol, Dept Mol Energet Microorganisms, Moscow 119899, Russia

来源：

FEBS LETTERS | 2004年 / 563卷 / 1-3期

基金：

俄罗斯基础研究基金会;

关键词：

sodium translocation; complex I; NADH dehydrogenase; Klebsiella pneumoniae; Vibrio;

D O I：

10.1016/S0014-5793(04)00312-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Properties of Klebsiella pneumoniae respiratory chain enzymes catalyzing NADH oxidation have been studied. Using constructed K. pneumoniae mutant strains, it was shown that three enzymes belonging to different families of NADH: quinone oxidoreductases operate in this bacterium. The NDH-2-type enzyme is not coupled with energy conservation, the NDH-1-type enzyme is a primary proton pump, and the NQR-type enzyme is homologous to the sodium-motive NADH dehydrogenase of Vibrio and is shown to be a primary Na+ pump. It is concluded that the NQR-type enzyme, not the NDH-1-type enzyme, catalyzes sodium-dependent NADH oxidation in K. pneumoniae. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：207 / 212

页数：6

共 33 条

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