Chemiluminescence detection of peroxynitrite with flow injection

被引：1

作者：

Kang, D ^{[1
]}

Zhou, YK ^{[1
]}

机构：

[1] Univ Ioannina, Dept Chem, Ioannina, Greece

来源：

INTERNATIONAL CONFERENCE ON SENSOR TECHNOLOGY (ISTC 2001), PROCEEDINGS | 2001年 / 4414卷

关键词：

peroxynitrite; nitric oxide; chemiluminescence; luminol; biosensor;

D O I：

10.1117/12.440163

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Peroxynitrie is a important derivative made by nitric oxide in vivo. It can make damages in many kinds of tissues and cells. its research value in heart diseases and cancer is very high. A sensitive, specific method for analysis of perxoynitrite is described. In this method, chemiluminescence. reaction between peroxynitrite and luminol was used to detect with flow injection system. The assay has a detection limit of 2x10(-8) mol L-1, and linear range of 5x10(-8)mol L-1 to 5x10(-5) mol L-1. The application of flow injection system offer possibility to establish biosensor for real-time detection of peroxynitrite.

引用

收藏

页码：104 / 107

页数：4

相关论文

共 16 条

[1]

Bauer G, 2000, ANTICANCER RES, V20, P4115

[2] APPARENT HYDROXYL RADICAL PRODUCTION BY PEROXYNITRITE - IMPLICATIONS FOR ENDOTHELIAL INJURY FROM NITRIC-OXIDE AND SUPEROXIDE [J].

BECKMAN, JS ;

BECKMAN, TW ;

CHEN, J ;

MARSHALL, PA ;

FREEMAN, BA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (04) :1620-1624

[3] OXIDATION OF NITRIC-OXIDE IN AQUEOUS-SOLUTION TO NITRITE BUT NOT NITRATE - COMPARISON WITH ENZYMATICALLY FORMED NITRIC-OXIDE FROM L-ARGININE [J].

IGNARRO, LJ ;

FUKUTO, JM ;

GRISCAVAGE, JM ;

ROGERS, NE ;

BYRNS, RE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :8103-8107

[4] SIMULATION OF THE DIFFUSION AND REACTION OF ENDOGENOUSLY PRODUCED NITRIC-OXIDE [J].

LANCASTER, JR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (17) :8137-8141

[5] INACTIVATION OF ALPHA-1-PROTEINASE INHIBITOR BY PEROXYNITRITE [J].

MORENO, JJ ;

PRYOR, WA .

CHEMICAL RESEARCH IN TOXICOLOGY, 1992, 5 (03) :425-431

[6] 2,7-Dihydrodichlorofluorescein diacetate as a fluorescent marker for peroxynitrite formation [J].

Possel, H ;

Noack, H ;

Augustin, W ;

Keilhoff, G ;

Wolf, G .

FEBS LETTERS, 1997, 416 (02) :175-178

[7] PEROXYNITRITE-INDUCED MEMBRANE LIPID-PEROXIDATION - THE CYTOTOXIC POTENTIAL OF SUPEROXIDE AND NITRIC-OXIDE [J].

RADI, R ;

BECKMAN, JS ;

BUSH, KM ;

FREEMAN, BA .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 288 (02) :481-487

[8]

Radi R, 1999, METHOD ENZYMOL, V301, P353

[9] The cardiovascular effects and implications of peroxynitrite [J].

Ronson, RS ;

Nakamura, M ;

Vinten-Johansen, J .

CARDIOVASCULAR RESEARCH, 1999, 44 (01) :47-59

[10] PEROXYNITRITE CAUSES APOPTOSIS IN RAT THYMOCYTES [J].

SALGO, MG ;

SQUADRITO, GL ;

PRYOR, WA .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 215 (03) :1111-1118