Deletion of the CD4 silencer element supports a stochastic mechanism of thymocyte lineage commitment

被引：66

作者：

Leung, RKM

Thomson, K

Gallimore, A

Jones, E

Van den Broek, M

Sierro, S

Alsheikhly, AR

McMichael, A

Rahemtulla, A

机构：

[1] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Med, Oxford OX3 9DU, England

[2] Univ Zurich, Inst Pathol, CH-8091 Zurich, Switzerland

[3] Univ Oxford, John Radcliffe Hosp, MRC, Human Immunol Unit, Oxford OX3 9DS, England

来源：

NATURE IMMUNOLOGY | 2001年 / 2卷 / 12期

基金：

英国惠康基金;

关键词：

D O I：

10.1038/ni733

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The mechanism of T cell lineage commitment remains controversial; to examine it we deleted the CD4-silencer element in the germ line of a mouse using a combination of gene targeting and Cre/LoxP-mediated recombination. We found that these mice were unable to extinguish CD4 expression either in immature thymocytes or mature CD8(+) cytotoxic T cells (CTLs), which resulted in the development of major histocompatibility complex class II-restricted double-positive CTLs in the periphery. This finding strongly supports a stochastic over an instructive mechanism of coreceptor down-regulation.

引用

收藏

页码：1167 / 1173

页数：7

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