Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements

被引：36

作者：

Neubauer, Stefan ^{[1
]}

Rugova, Ariana ^{[1
,2
]}

Chu, Dinh Binh ^{[1
]}

Drexler, Hedda ^{[1
]}

Ganner, Anja ^{[3
]}

Sauer, Michael ^{[4
,5
]}

Mattanovich, Diethard ^{[4
,5
]}

Hann, Stephan ^{[1
,5
]}

Koellensperger, Gunda ^{[1
,5
]}

机构：

[1] Univ Nat Resources & Life Sci BOKU, Dept Chem, Div Analyt Chem, A-1180 Vienna, Austria

[2] Univ Prishtina, Fac Math & Nat Sci, Dept Chem, Pristina 10000, Kosovo, Serbia

[3] Biomin Res Ctr, A-3430 Tulln, Austria

[4] Univ Nat Resources & Life Sci BOKU, Dept Biotechnol, A-1180 Vienna, Austria

[5] Austrian Ctr Ind Biotechnol ACIB, A-1180 Vienna, Austria

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2012年 / 404卷 / 03期

关键词：

Nucleotide; Nucleoside; Nucleobase; LC-MS-MS; LC-ICP-MS; Feed supplement; POROUS GRAPHITIC CARBON; LIQUID-CHROMATOGRAPHY; QUANTITATIVE-DETERMINATION; DNA-ADDUCTS; CAPILLARY-ELECTROPHORESIS; REACTION CELL; PHOSPHORUS; MS; QUANTIFICATION; METABOLITES;

D O I：

10.1007/s00216-012-6170-9

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection < 1 mu mol L-1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.

引用

页码：799 / 808

页数：10

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