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Determination of 18 nucleobases, nucleosides and nucleotides in human peripheral blood mononuclear cells by isocratic solvent-generated ion-pair chromatography

被引：13
作者：
Cichna, M
Daxecker, H
Raab, M
机构：
[1] Univ Vienna, Inst Analyt Chem, A-1090 Vienna, Austria
[2] Kaiser Franz Josef Hosp, Ludwig Boltzmann Inst Cardiothorac Res, Inst Lab Diagnost, A-1100 Vienna, Austria
来源：
ANALYTICA CHIMICA ACTA | 2003年 / 481卷 / 02期
关键词：
solvent generation; ion-pair chromatography; inosine 5 '-monophosphate dehydrogenase; mycophenolic acid; xanthosine 5 '-monophosphate; peripheral blood mononuclear cells;
D O I：
10.1016/S0003-2670(03)00081-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The paper describes a method for the separation of 18 nucleotides,nucleosides and nucleobases by isocratic solvent-generated ion-pair chromatography in 55 min. It has been developed for pharmacodynamic monitoring of mycophenolic acid, the active metabolite of the immunosuppressant mycophenolate mofetil. The method was applied for the detection of mycophenolic acid-induced changes in inosine 5'-monophosphate dehydrogenase (IMPDH) activity in the lysate of human peripheral blood mononuclear cells. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：245 / 253
页数：9
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