Simultaneous determination of lactate and pyruvate in human sweat using reversed-phase high-performance liquid chromatography: a noninvasive approach

被引：93

作者：

Biagi, Simona ^{[1
]}

Ghimenti, Silvia ^{[2
]}

Onor, Massimo ^{[3
]}

Bramanti, Emilia ^{[1
,3
]}

机构：

[1] UOS Pisa, Natl Res Council Italy, CNR, IPCF, I-56124 Pisa, Italy

[2] Dipartimento Chim & Chim Ind, I-56127 Pisa, Italy

[3] UOS Pisa, Natl Res Council Italy, CNR, ICCOM, I-56124 Pisa, Italy

来源：

BIOMEDICAL CHROMATOGRAPHY | 2012年 / 26卷 / 11期

关键词：

sweat analysis; reversed-phase chromatography; lactate; pyruvate; pyruvate ratio; FREE AMINO-ACIDS; HUMAN ECCRINE SWEAT; D-LACTIC ACID; BLOOD LACTATE; OXIDATIVE STRESS; LC-MS; EXERCISE; PLASMA; MUSCLE; URINE;

D O I：

10.1002/bmc.2713

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The simultaneous determination of lactate and pyruvate in sweat has been performed using reversed phase high-performance liquid chromatography (RP-HPLC) with UV detection at 220?nm. The calibration curves were linear in the investigated range 0.3 - 350?mm of lactate, 0.003- 1?mm of pyruvate. The sensitivity was good with a limit of detection of 0.03?mm for lactate and 0.001?mm for pyruvate. Recoveries evaluated for the entire procedure were 102?+/-?0.1 and 96?+/-?0.1for lactate and pyruvate, respectively. The method was successfully applied to analysis of sweat in 8 athletes at rest (pilocarpine sweating) and during physical exercise. Copyright (c) 2012 John Wiley & Sons, Ltd.

引用

页码：1408 / 1415

页数：8

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