New class of IMP cyclohydrolases in Methanococcus jannaschii

被引：18

作者：

Graupner, M ^{[1
]}

Xu, HM ^{[1
]}

White, RH ^{[1
]}

机构：

[1] Virginia Polytech Inst & State Univ, Dept Biochem 0308, Blacksburg, VA 24061 USA

来源：

JOURNAL OF BACTERIOLOGY | 2002年 / 184卷 / 05期

关键词：

D O I：

10.1128/JB.184.5.1471-1473.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The enzyme responsible for observed IMP cyclohydrolase activity in Methanococcus jannaschii was purified and sequenced: its genetic locus was found to correspond to gene MJ0626. The MJ0626 gene was cloned, and its protein product was expressed in Escherichia coli and shown to catalyze the cyclization of 5-formylamidoimidazole-4-carboxamide ribonucleotide to IMP. The enzyme has no sequence similarity to known enzymes, and its catalytic properties appear distinct from any characterized IMP cyclohydrolase. The purO gene for the enzyme is currently found only in the domain Archaea.

引用

页码：1471 / 1473

页数：3

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