Potency test of inactivated Newcastle disease vaccines by monoclonal antibody blocking ELISA

被引：5

作者：

Horváth, E

Czifra, G

Nagy, E

Engström, B

Mérza, M

机构：

[1] State Control Inst Vet Biol Drugs & Feeds, H-1475 Budapest, Hungary

[2] Natl Vet Inst, Dept Poultry, S-75007 Uppsala, Sweden

[3] Natl Vet Inst, SVANOVA Biotech, S-75183 Uppsala, Sweden

来源：

VACCINE | 1999年 / 17卷 / 23-24期

关键词：

inactivated Newcastle disease vaccine; potency test; monoclonal antibody blocking ELISA;

D O I：

10.1016/S0264-410X(99)00174-7

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The potency of 27 different inactivated Newcastle disease (ND) vaccines was determined by immunization and challenge tests, Three- and four-week-old SPF chickens were immunized with 1/50 dose of vaccines. The chickens were bled and challenged three weeks later, The protected/challenged ratio was determined, Serum samples were tested by the haemagglutination inhibition (HI) test and by a monoclonal antibody (MAB) blocking enzyme-linked immunosorbent assay (B-ELISA). Ninety-four percent of the vaccinated chickens (353 birds) with a B-ELISA value above 60% were protected. A strong positive correlation (0.934) was found between 'actual protection' (protection against challenge) and 'estimated protection' (protection calculated from the B-ELISA results). Based upon the results obtained, this B-ELISA seems to be suitable for replacing the challenge test in the potency control of inactivated ND vaccines in the future. (C) 1999 Elsevier Science Ltd. All rights reserved,

引用

页码：2969 / 2973

页数：5

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