A member of a new Tospovirus species isolated in Italy from wild buckwheat (Polygonum convolvulus)

被引：32

作者：

Ciuffo, M. ^{[1
]}

Tavella, L. ^{[2
]}

Pacifico, D. ^{[1
]}

Masenga, V. ^{[1
]}

Turina, M. ^{[1
]}

机构：

[1] CNR, Ist Virol Vegetale, Sez Torino, I-10135 Turin, Italy

[2] Univ Turin, Di Va PRA Entomol & Zool Applicate Ambiente, I-10095 Grugliasco, TO, Italy

来源：

ARCHIVES OF VIROLOGY | 2008年 / 153卷 / 11期

关键词：

D O I：

10.1007/s00705-008-0228-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Electron microscopy of extracts from diseased Polygonum convolvulus plants from Piedmont (Italy) revealed particles with the morphological features of a tospovirus. Sequencing of the full-length small (S) and medium (M) genome segments indicated that the virus is a member of a new Tospovirus species provisionally named Polygonum ringspot virus. A feature distinguishing it from members of other Tospovirus species was the presence of a very short intergenic region on the S segment lacking the potential for formation of the predicted hairpin structure involved in subgenomic expression. Antibodies made against purified nucleocapsids allowed serological comparison with other tospovirus isolates and revealed a relationship with tomato yellow ring virus, and to a lesser extent, to iris yellow spot virus. Serological tests detected the virus in various locations in northern and central Italy. The experimental host range was wide, although in nature the virus appeared restricted to two Polygonum species.

引用

页码：2059 / 2068

页数：10

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