A cytotoxic, apoptotic, low-molecular weight factor from pineal gland

被引:14
作者
Catrina, SB
Catrina, AI
Sirzén, F
Griffiths, W
Bergman, T
Biberfeld, P
Coculescu, M
Mutt, V
机构
[1] Univ Med & Pharm Carol Davila, Dept Endocrinol 2, Bucharest, Romania
[2] Univ Med & Pharm Carol Davila, SF Maria Hosp, Dept Rheumatol, Bucharest, Romania
[3] Karolinska Hosp, Radium Hemmet, Dept Oncol, S-10401 Stockholm, Sweden
[4] Karolinska Inst, S-17177 Stockholm, Sweden
[5] Karolinska Hosp, Immunopathol Lab, S-17176 Stockholm, Sweden
关键词
pineal; cancer; K562; apoptosis; purification;
D O I
10.1016/S0024-3205(99)00335-5
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Previous studies suggest that the pineal gland may play a, role in tumour growth inhibition. In this respect, melatonin, as the major hormone of this gland, has been extensively studied. However, there is growing evidence for the existence of other yet unknown pineal factors that may have tumour growth inhibiting properties. Here we describe the partial purification of a highly cytotoxic low molecular weight (<400 Da) hydrophilic fraction (designated F2M3R), starting from a porcine pineal extract (PE), via methanol precipitation followed by reverse-phase HPLC. F2M3R is cytotoxic for a highly apoptosis-resistant human erythroleukemia cell line (K562) at a concentration as low as 30 mu g/ml. The viability of the cells was not influenced by an identical prepared porcine pituitary extract or by melatonin. PE induces apoptosis in K562 cells as indicated by three different criteria: morphology, in situ TUNEL assay and bi-parametric FAGS analysis with annexin V and propidium iodide, but does not influence the viability of stimulated peripheral blood mononuclear cells. These observations warrant further purification and validation of the cytotoxicity in a panel of different human tumour and non-malignant cells.
引用
收藏
页码:1047 / 1057
页数:11
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