Impurities preparation of sodium tanshinone IIA sulfonate by high-speed counter-current chromatography and identification by liquid chromatography/multistage tandem mass spectrometry

被引：13

作者：

Chen, Shanqiao ^{[1
,2
,3
]}

Zhao, Xin ^{[1
,2
]}

Li, You ^{[1
,2
,3
]}

Yang, Dan ^{[1
,2
,3
]}

Zhou, Tingting ^{[1
,2
]}

Fan, Guorong ^{[1
,2
]}

机构：

[1] Shanghai Key Lab Pharmaceut Metabolite Res, Shanghai 200433, Peoples R China

[2] Second Mil Med Univ, Sch Pharm, Shanghai 200433, Peoples R China

[3] Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2013年 / 1288卷

关键词：

Sodium Tanshinone IIA Sulfonate; Impurity; Reference compounds; LC-MSn; Counter current chromatography; SALVIA-MILTIORRHIZA BUNGE; SEPARATION; MECHANISM; RAT;

D O I：

10.1016/j.chroma.2013.02.055

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Sodium Tanshinone IIA Sulfonate, the chemical modification product of Tanshinon IIA (STIIAS) is widely used in clinical treatment of angiocardiopathy, and the preparation of reference compounds of related substances for quality control is necessary. A method for separating three related substances from the bulk drug of STIIAS with high-speed counter-current chromatography (HSCCC) was developed. A solvent system composed of chloroform-n-butanol-methanol-water (17:0.3:14:9) was used and 0.5% saturated ammonium acetate aqueous solution was added to depress the emulsification in order to enhance sample loading capacity. Twelve related substances were identified in the bulk drug by HPLC-DAD and liquid chromatography/multistage tandem mass spectrometry (LC-MSn). Among them, three compounds were prepared and confirmed with H-1 NMR, one was identified without pretreatment, and seven components were identified after HSCCC enrichment since the contents of them were too low to be detected through direct LC-MSn injection. This research successfully broadened the application of HSCCC and introduced it to the field of impurities preparation of chemical medicine. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：28 / 34

页数：7

共 28 条

[1]

[Anonymous], 1984, J Tradit Chin Med, V4, P20

[2]

Effect of Supplementation of Tanshinone IIA and Sodium Tanshinone IIA Sulfonate on the Anticancer Effect of Epirubicin: An In Vitro Study [J].