Optimization of extracellular glucan production from Pleurotus eryngii and its impact on angiogenesis

被引：20

作者：

Shenbhagaraman, Ramalingam ^{[1
]}

Jagadish, Loganathan K. ^{[1
]}

Premalatha, Kishorekumar ^{[1
]}

Kaviyarasan, Venkatesan ^{[1
]}

机构：

[1] Univ Madras, Ctr Adv Studies Bot, Madras 600025, Tamil Nadu, India

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2012年 / 50卷 / 04期

关键词：

Extracellular polysaccharide; King oyster; Anti-angiogenesis; Box-Behnken; CAM assay; SUBMERGED CULTURE-CONDITIONS; STATISTICAL OPTIMIZATION; ANTRODIA-CINNAMOMEA; POLYSACCHARIDE PRODUCTION; MYCELIAL GROWTH; MUSHROOM; CULTIVATION; ANTITUMOR; BIOMASS; INHIBITION;

D O I：

10.1016/j.ijbiomac.2012.02.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Pleurotus eryngii, an edible mushroom with therapeutic potential was optimized using response surface methodology of four-factor Box-Behnken design for maximum mycelial biomass and extracellular glucan (EPS) production. The model predicts to gain a maximal mycelial biomass and extracellular polysaccharide at 39.4 g/l; 36.04 g/l of glucose, 8.27 g/l; 7.51 g/l of yeast extract, pH 6.99; 7.07 and temperature 26.2 degrees C; 25.84 degrees C, respectively. The validation experiments showed that the model was significant and in close agreement with the model prediction. The evaluation of extracellular polysaccharide on angiogenesis by ex vivo CAM assay showed that there was significant inhibition in neo-vascularization. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：957 / 964

页数：8

共 43 条

[1]

Mushrooms, tumors, and immunity: An update [J].

Borchers, AT ;

Keen, CL ;

Gershwin, ME .

EXPERIMENTAL BIOLOGY AND MEDICINE, 2004, 229 (05) :393-406

[2]

Ganoderma lucidum polysaccharides peptide inhibits the growth of vascular endothelial cell and the induction of VEGF in human lung cancer cell [J].

Cao, QZ ;

Lin, ZB .

LIFE SCIENCES, 2006, 78 (13) :1457-1463

[3]

A β-glucan from the fruit bodies of edible mushrooms Pleurotus eryngii and Pleurotus ostreatoroseus [J].

Carbonero, Elaine R. ;

Gracher, Ana Helena P. ;

Smiderle, Fhernanda R. ;

Rosado, Fabio R. ;

Sassaki, Guilherme L. ;

Gorin, Philip A. J. ;

Iacomini, Marcello .

CARBOHYDRATE POLYMERS, 2006, 66 (02) :252-257

[4]

Chang S. T., 2007, Micologia Aplicada International, V19, P33

[5]

Artesunate reduces chicken chorioallantoic membrane neovascularisation and exhibits antiangiogenic and apoptotic activity on human microvascular dermal endothelial cell [J].

Chen, HH ;

You, LL ;

Li, SB .

CANCER LETTERS, 2004, 211 (02) :163-173

[6]

Study for anti-angiogenic activities of polysaccharides isolated from Antrodia cinnamomea in endothelial cells [J].

Cheng, JJ ;

Huang, NK ;

Chang, TT ;

Wang, DL ;

Lu, MK .

LIFE SCIENCES, 2005, 76 (26) :3029-3042

[7]

A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].

DUBOIS, M ;

GILLES, K ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

NATURE, 1951, 168 (4265) :167-167

[8]

Yield, size and bacterial blotch resistance of Pleurotus eryngii grown on cottonseed hulls/oak sawdust supplemented with manganese, copper and whole ground soybean [J].

Estrada, A. E. Rodriguez ;

Royse, D. J. .

BIORESOURCE TECHNOLOGY, 2007, 98 (10) :1898-1906

[9]

VEGF: an update on biological and therapeutic aspects [J].

Farrara, N .

CURRENT OPINION IN BIOTECHNOLOGY, 2000, 11 (06) :617-624

[10]

Statistical optimization of media for mycelial growth and exo-polysaccharide production by Lentinus edodes and a kinetic model study of two growth morphologies [J].

Feng, Yi-Li ;

Li, Wei-Qi ;

Wu, Xue-Qian ;

Cheng, Jun-Wen ;

Ma, Su-Yun .

BIOCHEMICAL ENGINEERING JOURNAL, 2010, 49 (01) :104-112

← 1 2 3 4 5 →