Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals

被引:1084
作者
Eiraku, Mototsugu [1 ]
Watanabe, Kiichi [1 ]
Matsuo-Takasaki, Marni [1 ]
Kawada, Masako [1 ]
Yonemura, Shigenobu [2 ]
Matsumura, Michiru [1 ]
Wataya, Takafumi [1 ]
Nishiyama, Ayaka [1 ]
Muguruma, Keiko [1 ]
Sasail, Yoshiki [1 ]
机构
[1] RIKEN, Ctr Dev Biol, Organogenesis & Neurogenesis Grp, Kobe, Hyogo 6500047, Japan
[2] RIKEN, Ctr Dev Biol, Electron Microscope Lab, Kobe, Hyogo 6500047, Japan
关键词
D O I
10.1016/j.stem.2008.09.002
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Here, we demonstrate self-organized formation of apico-basally polarized cortical tissues from ESCs using an efficient three-dimensional aggregation culture (SFEBq culture). The generated cortical neurons are functional, transplantable, and capable of forming proper long-range connections in vivo and in vitro. The regional identity of the generated pallial tissues can be selectively controlled (into olfactory bulb, rostral and caudal cortices, hem, and choroid plexus) by secreted patterning factors such as Fgf, Wnt, and BMP. In addition, the in vivo-mimicking birth order of distinct cortical neurons permits the selective generation of particular layer-specific neurons by timed induction of cell-cycle exit. Importantly, cortical tissues generated from mouse and human ESCs form a self-organized structure that includes four distinct zones (ventricular, early and late cortical-plate, and Cajal-Retzius cell zones) along the apico-basal direction. Thus, spatial and temporal aspects of early corticogenesis are recapitulated and can be manipulated in this ESC culture.
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收藏
页码:519 / 532
页数:14
相关论文
共 43 条
[1]   Cortical calcium waves in resting newborn mice [J].
Adelsberger, H ;
Garaschuk, O ;
Konnerth, A .
NATURE NEUROSCIENCE, 2005, 8 (08) :988-990
[2]   Birth-date-dependent segregation of the mouse cerebral cortical neurons in reaggregation cultures [J].
Ajioka, I ;
Nakajima, K .
EUROPEAN JOURNAL OF NEUROSCIENCE, 2005, 22 (02) :331-342
[3]   Neuronal subtype-specific genes that control corticospinal motor neuron development in vivo [J].
Arlotta, P ;
Molyneaux, BJ ;
Chen, J ;
Inoue, J ;
Kominami, R ;
Macklis, JD .
NEURON, 2005, 45 (02) :207-221
[4]   Multiple origins of Cajal-Retzius cells at the borders of the developing pallium [J].
Bielle, F ;
Griveau, A ;
Narboux-Nême, N ;
Vigneau, S ;
Sigrist, M ;
Arber, S ;
Wassef, M ;
Pierani, A .
NATURE NEUROSCIENCE, 2005, 8 (08) :1002-1012
[5]   Pax6, Tbr2, and Tbr1 are expressed sequentially by radial glia, intermediate progenitor cells, and postmitotic neurons in developing neocortex [J].
Englund, C ;
Fink, A ;
Lau, C ;
Pham, D ;
Daza, RAM ;
Bulfone, A ;
Kowalczyk, T ;
Hevner, RF .
JOURNAL OF NEUROSCIENCE, 2005, 25 (01) :247-251
[6]   Restriction of late cerebral cortical progenitors to an upper-layer fate [J].
Frantz, GD ;
McConnell, SK .
NEURON, 1996, 17 (01) :55-61
[7]   Large-scale oscillatory calcium waves in the immature cortex [J].
Garaschuk, O ;
Linn, J ;
Eilers, J ;
Konnerth, A .
NATURE NEUROSCIENCE, 2000, 3 (05) :452-459
[8]   An intrinsic mechanism of corticogenesis from embryonic stem cells [J].
Gaspard, Nicolas ;
Bouschet, Tristan ;
Hourez, Raphael ;
Dimidschstein, Jordane ;
Naeije, Gilles ;
van den Ameele, Jelle ;
Espuny-Camacho, Ira ;
Herpoel, Adele ;
Passante, Lara ;
Schiffmann, Serge N. ;
Gaillard, Afsaneh ;
Vanderhaeghen, Pierre .
NATURE, 2008, 455 (7211) :351-U10
[9]  
Gonzalez JL, 1997, J NEUROSCI, V17, P9204
[10]   Cellular and molecular control of neurogenesis in the mammalian telencephalon [J].
Guillemot, F .
CURRENT OPINION IN CELL BIOLOGY, 2005, 17 (06) :639-647