An improved deblocking agent for direct Fmoc solid-phase synthesis of peptide thioesters

被引：31

作者：

Bu, XZ

Xie, GY

Law, CW

Guo, ZH ^{[1
]}

机构：

[1] Hong Kong Univ Sci & Technol, Dept Chem, Kowloon, Hong Kong, Peoples R China

[2] Hong Kong Univ Sci & Technol, Biotechnol Res Ctr, Kowloon, Hong Kong, Peoples R China

来源：

TETRAHEDRON LETTERS | 2002年 / 43卷 / 13期

关键词：

D O I：

10.1016/S0040-4039(02)00237-X

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

To synthesize peptide thioesters directly on a solid support for use as substrate analogues for thioesterases in non-ribosomal peptide synthases, we modified a reagent compatible with Fmoc solid-phase peptide synthesis that efficiently removes the protecting group while keeping the thioester intact. The deprotecting reagent, consisting of DBU and HOBt, was successfully used to prepare a decapeptide in high yield. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：2419 / 2422

页数：4

共 14 条

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