Regulation of the expression of the tissue transglutaminase gene by DNA methylation

被引：53

作者：

Lu, S ^{[1
]}

Davies, PJA ^{[1
]}

机构：

[1] UNIV TEXAS, SCH MED, DEPT PHARMACOL, HOUSTON, TX 77225 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 09期

关键词：

D O I：

10.1073/pnas.94.9.4692

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have investigated the role of DNA methylation in the regulation of the expression of the human tissue transglutaminase gene. Studies on the methylation of the transglutaminase promoter in normal and neoplastic human cells demonstrated that the promoter is methylated in vivo and hypomethylation of the promoter is correlated with constitutive gene expression. Demethylation of the promoter in vivo by treatment of the cells with 5-azacytidine increased transglutaminase expression and hypermethylation of the promoter in vitro suppressed its activity. These studies suggest that alternations in DNA methylation mag be one of the mechanisms regulating the tissue-specific expression of the tissue transglutaminase gene.

引用

页码：4692 / 4697

页数：6