Long time monitoring of the respiratory activity of isolated mitochondria

被引：7

作者：

Heller, Anne ^{[1
]}

Fischer, Lorenz H. ^{[2
]}

Wolfbeis, Otto S. ^{[2
]}

Goepferich, Achim ^{[1
]}

机构：

[1] Univ Regensburg, Dept Pharmaceut Technol, D-93053 Regensburg, Germany

[2] Univ Regensburg, Inst Analyt Chem Chemo & Biosensors, D-93053 Regensburg, Germany

来源：

EXPERIMENTAL CELL RESEARCH | 2012年 / 318卷 / 14期

关键词：

Isolated mitochondria; Respiratory activity; Optical oxygen sensor; High throughput screening; HUMAN MYOCARDIAL-CELLS; OXYGEN; CANCER; DRUG; DISEASE; ASSAYS;

D O I：

10.1016/j.yexcr.2012.05.007

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have investigated the ability of optical oxygen sensors incorporated in a microplate to determine the respiratory activity of cell fractions. Different cell fractions were monitored, in particular to evaluate the long term functionality of isolated mitochondria. It is possible to continuously sense respiratory activity of isolated mitochondria over time. We found that they are functional for three hours but stop respiring at a critical limit of 20% air saturation in the system. Furthermore, inhibition and enhancement of respiratory activity were detected. In conclusion, oxygen sensors are a powerful tool to evaluate the functionality of isolated mitochondria. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：1667 / 1672

页数：6

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